Talent qPCR PreMix (SYBR Green) Featured Image

Luso qPCR PreMix (SYBR Green)

Kukana kwabwino kusokonezedwa ndi chodetsa komanso kufulumira kwa ma templates ovuta.

Chogulitsa ichi ndi reagent yapadera ya Real-Time PCR yogwiritsa ntchito SYBR Green I chimeric fluorescence. The 2 × Talent qPCR PreMix imagwiritsa ntchito anti Taq DNA polymerase, ndipo makina apadera a qPCR Buffer amatha kuwonetsetsa kuti malonda akugwira bwino ntchito komanso mwachangu pa qPCR pazida zonse za Real-Time PCR. Nthawi yochitira qPCR ikhoza kufupikitsidwa ndi 50%. Kuphatikiza apo, kuwonjezera kwa H-Competitor factor ndi EP gawo mu Buffer kumapangitsa kuti malonda akhale ndi mitundu yambiri yazitsanzo. Ili ndi kugwiritsa ntchito bwino ma templates okhala ndi zovuta zapamwamba, ma tempuleti okhala ndi zotsalira zambiri za PCR zoletsa, kukweza kwa zidutswa zazitali, ndi zina zambiri.
Chogulitsidwacho chimakhalanso ndi mawonekedwe amakulidwe apamwamba, makulitsidwe apamwamba, osiyanasiyana odalirika, ndi zina zambiri, ndipo amatha kupeza zotsatira mwachangu osakhudza zotsatira za PCR.

Mphaka. Ayi	Kukulitsa Kukula
4992882	20 ×l × 125 rxn
4992775	20 ×l × 500 rxn
4992866	20 ×l × 5000 rxn

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Asitikali ofunikira kwambiri omwe amasinthidwa ma polymerase amatha kufupikitsa nthawi ndi theka.
■ H-Mpikisano amapikisana ndi hydrogen bond, yomwe imatsimikizira kukweza kwama templates okhala ndi GC yambiri, kapangidwe kake kosavuta ndi ma templates ataliatali.
■ Gawo la EP limakhazikitsa dongosolo la PCR, limateteza moyenera ntchito ya enzyme komanso limakana kusokonezedwa ndi zoletsa zosiyanasiyana.
■ Phukusi lopanda utoto limalowa m'malo mwa chubu labulauni, kuti zotsalazo ziwonekere.

Mapulogalamu

Ndioyenera kusanthula mafotokozedwe, kudziwa kwa acidic acid ndi mitundu ina ya zoyeserera pazida zenizeni za PCR ndi njira ya SYBR Green, makamaka kwa GC yapamwamba, kapangidwe kake kovuta, zotsalira zodetsa kwambiri komanso kuchuluka kwa template yayitali.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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Experimental Example

Mtundu wa Human UH11 (GC: 73.4%) udatsimikizidwa ndi TIANGEN Talent qPCR PreMix (kumanzere) ndi chinthu chofunikira kuchokera kwa Supplier A (pakati) ndi B (kumanja). Zotsatira zikuwonetsa kuti poyerekeza ndi omwe amapereka, Talent qPCR PreMix ili ndi ma curveit owoneka bwino a ma gradients 5 osanjikiza, kutsika kwa Ct, kukwera kwa kuwala kwa fluorescence, ndi chimango chimodzi chosungunuka, zomwe zikuwonetsa kuti Talent qPCR PreMix ili ndimasinthidwe abwino komanso mphamvu yakukweza pama templates apamwamba a GC.

Lembani uthenga wanu apa ndi kutumiza kwa ife