FastFire qPCR PreMix (Probe) Featured Image

FastFire qPCR PreMix (Kafukufuku)

Kafukufuku wofulumira kwambiri wowonjezera reagent.

FastFire qPCR PreMix (Probe) imagwiritsa ntchito anti anti anti anti Taq DNA polymerase ndi pulogalamu yapadera yofulumira ya PCR Buffer kuti muwonetsetse chidwi cha qPCR pazida zonse za Real-Time PCR. Ili ndi mawonekedwe a kuchitapo kanthu mwachangu, ndi 60% yochepa ya PCR reaction time, kukweza kwakukulu, kukweza kwapamwamba kwambiri komanso mitundu yonse yodalirika, zomwe zimathandizira kusonkhanitsa zotsatira mwachangu popanda kukhudza zotsatira za PCR.

Mphaka. Ayi	Kukulitsa Kukula
4992220	20 ×l × 125 rxn
4992221	20 ×l × 500 rxn

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Mofulumira: Antibody yosinthidwa Anti Taq DNA polymerase, yogwirizana ndi makina osanja mwachangu, yopulumutsa mpaka 60% ya nthawi yogwirira ntchito ndikukhala wofufuza mwachangu pamsika pakadali pano.
■ Kutha kwamphamvu kwamphamvu: Chizindikiro champhamvu cha fluorescence, zotsatira zolondola kwambiri komanso zodalirika.
■ Kukhazikika kwabwino: Kukhazikika kwa PCR kolimbitsa thupi ndikuwonjezera zina kumawonjezeredwa ku buffer kuti zotsatira zake zizikhala zokhazikika, zobwereza, zolondola komanso zodalirika.
■ Kugwiritsa ntchito kwambiri: Sikokwanira kugwiritsa ntchito chida chenicheni cha PCR, komanso kuyeneranso kugwiritsa ntchito chida chenicheni cha PCR.
Kukonzekera kwa ROX: Utoto wa ROX umaphatikizidwa padera, womwe umakhala wosavuta kugwiritsa ntchito ndipo umakhala ndi zotsatira zolondola.

Mfundo

Mtundu: DNA yoyambitsa yotentha yotulutsa ma cell polymerase, kafukufuku.
Liniya osiyanasiyana: 10⁰-10⁷
Nthawi yogwiritsira ntchito: ~ 20 min
Mapulogalamu:Kafukufuku wochokera ku qPCR wopeza majini pazitsanzo za DNA kuchokera m'malo osiyanasiyana. Multiplex PCR ikupezeka.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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	Mwachangu- Sinthani kuyeserera koyeserera, onjezerani moyo wautumiki wa chida ndikuwononga mphamvu Anti Taq DNA polymerase itha kuyambitsidwa mwachangu. Kuphatikiza ndi dongosolo la Buffer lapadera, pakadali pano ndi Probe reagent wofulumira kwambiri pamsika. Kufufuza kofulumira kwambiri pakadali pano, komwe kumachepetsa nthawi yochitira ndi 1000 sec.
	Kutalika kotalikira kotheka Chogulitsidwacho chili ndi mawonekedwe osiyanasiyana. Ikhoza kuzindikira ma tempuleti otsika ngati 0.1 pg / μl, okhala ndi kukweza kwakukulu, kubwereza kwabwino komanso ubale wabwino kwambiri.Gwiritsirani ntchito cDNA ya anthu monga template, kusungunuka kwakanthawi kambiri mpaka ma gradients 7 (ndende kuchokera ku 100 ng / μl mpaka 0.1 pg / μl) .