FastFire qPCR PreMix (SYBR Green)

Njira yofulumira kwambiri ya SYBR Green fluorescent reagent.

FastFire qPCR PreMix imagwiritsa ntchito anti antiqq Taq DNA polymerase ndi PCR Buffer yoyeserera yapadera kuti iwonetsetse chidwi cha qPCR pazida zonse za Real-Time PCR. Ili ndi mawonekedwe a kuchitapo kanthu mwachangu, ndi 60% yochepa ya PCR reaction time, kukweza kwakukulu, kukweza kwapamwamba kwambiri komanso mitundu yonse yodalirika, zomwe zimathandizira kusonkhanitsa zotsatira mwachangu popanda kukhudza zotsatira za PCR.

Mphaka. Ayi	Kukulitsa Kukula
4992217	20 ×l × 125 rxn
4992249	20 ×l × 5000 rxn
4992218	20 ×l × 500 rxn

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Chofulumira kwambiri: Antibody yosinthidwa Anti Taq DNA polymerase, yogwirizana ndi makina osanja mwachangu, yopulumutsa mpaka 60% ya nthawi yogwirira ntchito ndikukhala othamanga kwambiri pa SYBR Green pamsika pakadali pano.
■ Kutha kwamphamvu kwamphamvu: Chizindikiro champhamvu cha fluorescence, zotsatira zolondola kwambiri komanso zodalirika.
■ Kukhazikika kwabwino: Kukhazikika kwa PCR kolimbitsa thupi ndikuwonjezera kumawonjezeredwa ku Buffer, ndikupangitsa zotsatira zake kukhala zokhazikika, zobwereza, zolondola komanso zodalirika.
■ Kugwiritsa ntchito kwambiri: Sikuti zimangoyenera kugwiritsa ntchito zida zenizeni za PCR, komanso zida zogwiritsira ntchito nthawi yeniyeni ya PCR.
Kukonzekera kwa ROX: Utoto wa ROX umaphatikizidwa padera, womwe umakhala wosavuta kugwiritsa ntchito ndipo umatha kutsimikizira zotsatira zolondola.

Mfundo

Mtundu: DNA-polymerase yosinthidwa ndi oteteza thupi, SYBR Green I
Nthawi yogwira ntchito: ~ 30 min

Kuyika: QPCR yochokera ku utoto wopeza majini pa DNA kapena kusanthula kwamawu pamagulu a mitundu ya cDNA

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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Ena: FastFire qPCR PreMix (Kafukufuku)

	Kutha kwamphamvu kwamphamvu- Chizindikiro cholimba cha fluorescence Chofulumira-Kuthandizani kuyeserera koyeserera, kutalikitsa moyo wautumiki wa chida ndikuwononga mphamvu Taq DNA polymerase itha kuyambitsidwa mwachangu, kuwonetsetsa kuti zinthu za FastFire zikhale zotsogola kwambiri pa SYBR Green pamsika pakadali pano ndi njira yapadera yolimbitsa Buffer kuti ikwaniritse kuyeserera koyeserera.
	Kutha kwamphamvu kwamphamvu- Chizindikiro cholimba cha fluorescence Chizindikiro cha fluorescence cholimba chimakhala champhamvu (kuthekera kokulitsa ndikulimba), kokhala ndi mawonekedwe okwera kwambiri, kukhudzika kwakukulu, ndipo imatha kuzindikira molondola komanso molondola kuchuluka kwa mtundu wa template yotsika kwambiri. Chizindikiro chazinthu zoyenera kuchokera kwa Supplier T ndi chofooka, ndipo mtengo wa CT wabwerera m'mbuyo, zomwe zitha kubweretsa zotsatira zolakwika.
	Kuyesa mobwerezabwereza kwa 96 kumawonetsa kuti reagent imakhala ndi kukhazikika kwamphamvu komanso kubwereza bwino.