EndoFree Mini Plasmid Kit II

Kuyeretsa kupatsirana kwa endotoxin wopanda plasmid DNA.

EndoFree Mini Plasmid Kit II imagwiritsa ntchito ukadaulo wapadera wa silika membrane kutsatsa bwino ndikugwira bwino plasmid DNA. Njira yapadera yochotsera endotoxin imatha kuchotsa zodetsa monga endotoxin ndi mapuloteni. Njira yonse yochotsera imatenga ola limodzi lokha, lomwe ndilosavuta komanso mwachangu. Zokolola za plasmid ndizokhudzana ndi kuchuluka kwa plasmid, mtundu wa mabakiteriya ndi chikhalidwe. Mpaka 70 μg wa plasmid DNA atha kutulutsidwa kuchokera ku 5-15 ml ya mabakiteriya otukuka usiku wonse.

Mphaka. Ayi	Kukulitsa Kukula
4992422	50 preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Zofulumira komanso zokolola zambiri: 5-70 μg plasmid DNA itha kutulutsidwa pafupifupi ola limodzi.
■ Kuyera kwambiri: Njira yapadera yochotsera endotoxin yophatikizidwa ndi Spin Column CP4 itha kutsatsa makamaka acidic acid ndikuchotsa endotoxin pamlingo wochepa.
■ Ntchito zingapo: Zoyenera kuyesa kwanthawi zonse monga choletsa endonuclease chimbudzi, PCR, kusanja, ligation, kusintha, komanso zoyeserera mwatsatanetsatane monga jini, cellinjection ya cell, kutseka kwa majini, kusindikiza, ndi zina zambiri.

Zokolola za Plasmid

Plasmid Yield

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

Previous: Zamgululi Chida cha EndoFree Maxi Plasmid

Ena: TIANprep N96 Maginito Plasmid zida

Oyenera kutulutsa ma plasmids okwera / otsika
Chithunzi 1. Kutulutsidwa kwa plasmid DNA kuchokera kumtundu wosiyanasiyana wama bacteria pogwiritsa ntchito EndoFree Mini Plasmid Kit II.
Kuchepetsa mphamvu: 200 μl; Kutsegula voliyumu: 3 μl otsika-plasmid pBR322, 2 μl makope apamwamba a plasmid pBS.
Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose.
Kutenga kwa selo ndi plasmid yoyeretsedwa

Chithunzi 2 PEGFP plasmid yoyeretsedwa ndi EndoFree Mini Plasmid Kit II idasinthidwa kukhala maselo a 293T. Kufotokozera kwa GFP kunapezeka mu 48 h pambuyo poti mutenge

Lembani uthenga wanu apa ndi kutumiza kwa ife