Chida cha EndoFree Maxi Plasmid

Kuyeretsa kupatsirana kwa endotoxin wopanda plasmid DNA.

Chida cha EndoFree Maxi Plasmid chimapangidwa ndi ukadaulo wa silika kuti atenge DNA ya plasmid yopanda endotoxin. Chida ichi chimapereka njira yapadera yochotsera endotoxin kuti apange ma plasmid apamwamba kwambiri a ntchito zotsikira. Amapangidwa kuti apatule plasmid DNA yoyera kwambiri kuchokera ku 100-200 ml ya chikhalidwe cha bakiteriya ndipo imatha kupereka 1.5 mg transasmion grade plasmid DNA.

Mphaka. Ayi Kukulitsa Kukula
4992194 10 ma preps

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Zokolola mwachangu: 200 μg-1.5 mg plasmid DNA imatulutsa ola limodzi lokhala ndi mapangidwe abwino kwambiri.
■ Kuyera kwambiri: DNA yoyera kwambiri ya plasmid imapezeka ndi gawo lapadera lotetezera ndi Spin Column CP6.
■ Kuchita bwino kwambiri kosandulika: Koyenererana ndi kuyeserera koyesera kwamizere yambiri yama cell.
■ Mapulogalamu osiyanasiyana: Oyenera kuyimitsa chimbudzi cha enzyme, kusintha, kutsatira, microinjection, kutseketsa majini ndi kuyeserera.

Zokolola za Plasmid

Plasmid Yield

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)


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    Experimental Example Kutulutsa kwapamwamba / kotsika kwa plasmid
    Plasmid pBR322 yotsika kwambiri, yotengedwa kuchokera ku 200 ml chikhalidwe cha bakiteriya pogwiritsa ntchito EndoFree Maxi Plasmid Kit, idasankhidwa mu 1 ml Buffer TB, yokhala ndi 0.6 μg / μl. Mapulogalamu apamwamba kwambiri a plasmid pBS, otengedwa kuchokera ku 100 ml ya chikhalidwe cha bakiteriya pogwiritsa ntchito EndoFree Maxi Plasmid Kit, adasankhidwa mu 1 ml Buffer TB, yokhala ndi 1.2 μg / μl.
    MIV: TIANGEN DNA Chizindikiro IV
    pBR322: 2 μl, pBS: 2 μl
    Experimental Example PEGFP yopezeka ndi EndoFree Maxi Plasmid Kit idasinthidwa padera kukhala endotoxin-insensitive cell line 293T ndi cell enditivexin cell cell Huh7 cell ndi TIANGEN TIANfect. Kufotokozera kwa GFP kunapezeka pakadutsa maola 24 mutapititsidwa.
    Lembani uthenga wanu apa ndi kutumiza kwa ife