TIANprep Rapid Mini Plasmid Kit

Pofuna kuyeretsa mwachangu kwa plasmid DNA yam'magulu a biology ndi njira ya alkaline lysis.

TIANprep Rapid Mini Plasmid Kit idakonzedwa kuchokera kuukadaulo wamankhwala wamchere wamchere, womwe DNA ya plasmid wapamwamba kwambiri imatha kuyeretsedwa mkati mwa mphindi 8. Chosungira chatsopano cha lysis chimalola kukhazikitsidwa kwa DNA pakhungu la silika pamaso pamchere wambiri. Zinthu zomwe nembanemba za silika zimapangidwa ndizapadera, zothandiza kwambiri komanso zachindunji. Chida ichi chakonzedwa kuti chizitsuka mpaka 24 μg plasmid DNA kuchokera ku 1-4 ml usiku wonse zikhalidwe zaE.coli. DNA ya plasmid yoyeretsedwa ndi chida ichi itha kugwiritsidwa ntchito poyesera mosiyanasiyana, monga chimbudzi cha enzyme, PCR, kusanja, ligation, kusintha, kuwunikira laibulale, kusindikiza kwa vitro ndikusinthitsa kwa maselo wamba azikhalidwe, ndi zina zambiri.

Mphaka. Ayi	Kukulitsa Kukula
4992191	50 preps
4992192	200 preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Kayendedwe

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Mwachangu: Njira yosavuta yopanda masitepe otsuka DNA ya plasmid yoyera mkati mwa mphindi 8 zokha.
■ Kuchita bwino kwambiri: Itha kuyeretsa zoposa 85% ya plasmid DNA kuchokera E.coli.
■ Chizindikiro cha utoto TIANRed chidaperekedwa kuti chiwongolere ndikukumbutsa chilichonse panthawi yoyesera kuti zitsimikizidwe kuti plasmid imadziyeretsa bwino.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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Ena: Chida cha EndoFree Midi Plasmid

Ndi chithandizo champhamvu kwambiri cha lysate komanso dongosolo lokhazikika, TIANprep Rapid Mini Plasmid Kit imatha kupeza zinthu zapamwamba kwambiri posunga nthawi yambiri yogwira ntchito.

Kuyerekeza pakati pa TIANprep Rapid Mini Plasmid Kit ndi Supplier A (general plasmid DNA kuyeretsa zida) poyeretsa plasmid yemweyo nthawi yomweyo.

Zitsanzo zamphamvu: 3 ml chikhalidwe chamadzulo cha E. coli (OD600 = 1.8); Kuchepetsa mphamvu: 50 μl; Kutsegula voliyumu: 1 μl pBS, 3 μl pET30a, 3 μl pBR322.

Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose gel. M: TIANGEN DNA Chizindikiro IV.

Kutsiliza: Agarose gel electrophoresis ikuwonetsa kuti TIANprep Rapid Mini Plasmid Kit itha kupeza zokolola zofananira za plasmid DNA monga momwe zimapangidwira kuyeretsa kwa plasmid mkati mwa 8 min.

TIANprep Rapid Mini Plasmid Kit