Chida cha EndoFree Midi Plasmid

Kuyeretsa mwachangu kwa sing'anga kuchuluka kwa endotoxin-free transfection grade plasmid DNA.

EndoFree Midi Plasmid Kit imagwiritsa ntchito njira yapadera yolumikizira ulusi kuti ichite bwino ndikumanga DNA ya plasmid. Buffer EBT yapadera ndi Filtration CS1 imatha kuchotsa zosafunika monga endotoxin ndi protein. Njira yonse yochotsera imatenga ola limodzi lokha, lomwe ndi losavuta komanso lofulumira.

Mphaka. Ayi Kukulitsa Kukula
4992853 10 ma preps

Mankhwala Mwatsatanetsatane

Kayendedwe

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Yoyenera kutulutsa ma plasmid kuchokera ku 50-100 ml ya mabakiteriya achikhalidwe.
■ Plasmid yoyeretsedwa imakhala ndi endotoxin yotsika kwambiri ndipo imatha kukwaniritsa zofunikira zakupatsirana kwapamwamba kwambiri.
■ Ndiosavuta kuyigwiritsa ntchito pogwiritsa ntchito njira yapadera komanso yofulumira yochotsera endotoxin.

Mapulogalamu

Plasmid DNA yoyeretsedwa itha kugwiritsidwa ntchito poyesa maselo a biology ndi zoyeserera zama cell cell monga choletsa endonuclease chimbudzi, kusanja, kusintha, kusintha, ma microinjection, ndi zina zambiri.

Zokolola za Plasmid

Yield

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)


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    Workflow

    Workflow Plasmid pEGFP-C1 imatsukidwa ndi EndoFree Midi Plasmid Kit kuchokera ku 50 ml usiku umodzi mabakiteriya otukuka. Kuchuluka kwa plasmid kunayezedwa ndi Nanodrop ndipo zokolola zake zimawerengedwa. Zotsatira zake zikuwonetsa kuti zokolola za plasmid zopangidwa ndi EndoFree Mini Plasmid Kit ndizokwera kuposa zomwe zimapangidwa ndi Wogulitsa A.
    Workflow Plasmid pEGFP-C1 yotulutsidwa ndi EndoFree Midi Plasmid Kit imasinthidwa kukhala 80% confluence 293T cell ndi TIANGEN TIANfect. Kufotokozera kwa GFP kunapezeka 24-36 h pambuyo pa kupatsidwa magazi. Zotsatira zake zikuwonetsa kuti magwiridwe antchito anali 80% -90%.
    Lembani uthenga wanu apa ndi kutumiza kwa ife