■ Yoyenera kutulutsa ma plasmid kuchokera ku 50-100 ml ya mabakiteriya achikhalidwe.
■ Plasmid yoyeretsedwa imakhala ndi endotoxin yotsika kwambiri ndipo imatha kukwaniritsa zofunikira zakupatsirana kwapamwamba kwambiri.
■ Ndiosavuta kuyigwiritsa ntchito pogwiritsa ntchito njira yapadera komanso yofulumira yochotsera endotoxin.
Plasmid DNA yoyeretsedwa itha kugwiritsidwa ntchito poyesa maselo a biology ndi zoyeserera zama cell cell monga choletsa endonuclease chimbudzi, kusanja, kusintha, kusintha, ma microinjection, ndi zina zambiri.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
Plasmid pEGFP-C1 imatsukidwa ndi EndoFree Midi Plasmid Kit kuchokera ku 50 ml usiku umodzi mabakiteriya otukuka. Kuchuluka kwa plasmid kunayezedwa ndi Nanodrop ndipo zokolola zake zimawerengedwa. Zotsatira zake zikuwonetsa kuti zokolola za plasmid zopangidwa ndi EndoFree Mini Plasmid Kit ndizokwera kuposa zomwe zimapangidwa ndi Wogulitsa A. | |
Plasmid pEGFP-C1 yotulutsidwa ndi EndoFree Midi Plasmid Kit imasinthidwa kukhala 80% confluence 293T cell ndi TIANGEN TIANfect. Kufotokozera kwa GFP kunapezeka 24-36 h pambuyo pa kupatsidwa magazi. Zotsatira zake zikuwonetsa kuti magwiridwe antchito anali 80% -90%. |
Chiyambireni kukhazikitsidwa, fakitole yathu yakhala ikupanga zinthu zoyambirira motsatira mfundoyo
yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..