TIANprep Rapid N96 Plasmid Kit

Kutulutsa kwakukulu, kutulutsa mwachangu pang'ono kwa plasmid.

Chikwamacho chimatengera njira ya isopropanol mpweya kuti ipeze plasmid DNA. Chikwamacho chimatengera mbale yazosefera 96 ​​komanso N ndi Buffer III, zomwe zimapangidwa ndi TIANGEN, ndipo zimatha kutulutsa DNA yoyera mwapamwamba kwambiri. Ndioyenera kukonzekera mwachangu ma plasmid apamwamba. 1.3 ml ya bakiteriya wamtundu wa plasmid wapamwamba kwambiri amatha kukonzedwa bwino, ndipo zitsanzo za 96 za plasmid DNA zimatulutsidwa mwachangu. Zokolola zambiri pachitsime ndi 10-20 μg (zokololazo ndizokhudzana ndi zinthu monga nambala ya plasmid, boma la bakiteriya, ndi zina zambiri). Plasmid DNA yotulutsidwa ndi zida zitha kugwiritsidwa ntchito poyesa ma biology ofufuza monga kuletsa kugaya kwa enzyme, kusanja, kuwunikira laibulale, ligation ndi kusintha.