■ Zokolola mwachangu: 200 μg-1.5 mg plasmid DNA imatulutsa ola limodzi lokhala ndi mapangidwe abwino kwambiri.
■ Kuyera kwambiri: DNA yoyera kwambiri ya plasmid imapezeka ndi gawo lapadera lotetezera ndi Spin Column CP6.
■ Kuchita bwino kwambiri kosandulika: Koyenererana ndi kuyeserera koyesera kwamizere yambiri yama cell.
■ Mapulogalamu osiyanasiyana: Oyenera kuyimitsa chimbudzi cha enzyme, kusintha, kutsatira, microinjection, kutseketsa majini ndi kuyeserera.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
Kutulutsa kwapamwamba / kotsika kwa plasmid Plasmid pBR322 yotsika kwambiri, yotengedwa kuchokera ku 200 ml chikhalidwe cha bakiteriya pogwiritsa ntchito EndoFree Maxi Plasmid Kit, idasankhidwa mu 1 ml Buffer TB, yokhala ndi 0.6 μg / μl. Mapulogalamu apamwamba kwambiri a plasmid pBS, otengedwa kuchokera ku 100 ml ya chikhalidwe cha bakiteriya pogwiritsa ntchito EndoFree Maxi Plasmid Kit, adasankhidwa mu 1 ml Buffer TB, yokhala ndi 1.2 μg / μl. MIV: TIANGEN DNA Chizindikiro IV pBR322: 2 μl, pBS: 2 μl |
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PEGFP yopezeka ndi EndoFree Maxi Plasmid Kit idasinthidwa padera kukhala endotoxin-insensitive cell line 293T ndi cell enditivexin cell cell Huh7 cell ndi TIANGEN TIANfect. Kufotokozera kwa GFP kunapezeka pakadutsa maola 24 mutapititsidwa. |
Chiyambireni kukhazikitsidwa, fakitole yathu yakhala ikupanga zinthu zoyambirira motsatira mfundoyo
yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..