■ Zizindikiro zamtundu zimawonjezeredwa kwa ma reagents kuti apewe zolakwika zowonjezera kapena kusiya chifukwa cha kuchuluka kwa zitsanzo.
■ Mtengo wa kuwala kwa fluorescence, chidwi chachikulu komanso kudziwa zambiri.
■ Atazindikira mosamalitsa, chizindikirocho sichingakhudze kufunika kwa kuwala kwa dzuwa ndi zotsatira zake zowonjezera za utoto.
■ Zoyenera pazida zosiyanasiyana za PCR (Roche, Bio-rad, ABI, ndi zina zambiri).
Ndioyenera mitundu yosiyanasiyana ya zoyeserera monga kusanthula kwamawu ndi kuzindikira kwa asidi ya asidi ndi njira ya SYBR Green pazida zosiyanasiyana za PCR. Ndizoyenera makamaka pakuyesa kwapamwamba kwambiri ndi zitsanzo zambiri komanso zovuta kuzitsatira.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
Chithunzi. 1. Ma graph a ma amplifi cation curves ndi ma curve osungunuka omwe amapezeka motsatana kuti awonetse mtundu wa HS1 wa anthu pogwiritsa ntchito TIANGEN SuperReal PreMix Plus (SYBR Green) (A) ndi SuperReal PreMix Colour (SYBR Green) (B). Chithunzicho chimasungunuka kwama gradients 5 ozungulira. Makhalidwe a Ct (C) omwe amapezeka poyerekeza akuwonetsa kuti kukulitsa kwa zida ziwirizi kumakhala ndi chidwi, kulunjika komanso kukhazikika. Kuyesaku kunayendetsedwa pa Roche LightCycler480. | |
Chithunzi 2. Ma graph a curve amplve ndi melting curve omwe amapezeka potengera mtundu wa HS5 wa anthu pogwiritsa ntchito TIANGEN SuperReal PreMix Colour (SYBR Green) ndi chinthu chofunikira kuchokera ku Supplier T (A: TIANGEN, B: Supporter T. ndipo yerekezerani zamtengo wapatali wa Ct (C) .Zotsatira zake zikuwonetsa kuti SuperReal PreMix Colour (SYBR Green) ili ndi chidwi chachikulu, chodziwika bwino komanso chokhazikika. Kuyesaku kunayendetsedwa pa Bio-Rad CFX96. |
Chiyambireni kukhazikitsidwa, fakitole yathu yakhala ikupanga zinthu zoyambirira motsatira mfundoyo
yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..