Maginito Seramu / Madzi a m'magazi DNA Maxi Kit

Oyenera kutulutsa DNA kuchokera ku 2-5 ml ya seramu / plasma yokhala ndi matulukidwe apamwamba.

Chikwamacho chimagwiritsa ntchito mikanda yamaginito yokhala ndi magwiridwe apadera opatukana komanso njira yapadera yotetezera kupatulira ndi kuyeretsa DNA yaulere yapamwamba kwambiri pazitsanzo monga seramu ndi plasma yokhala ndi 2-5 ml. Mikanda yamaginito yolumikizidwa mwapadera imagwirizana kwambiri ndi asidi ya asidi munthawi zina. Zinthu zikasintha, maginito amatulutsa ma adsorbed nucleic acid, potero amakwaniritsa cholinga chodzipatula mwachangu komanso kuyeretsa asidi wa asidi. Njira yonseyi ndiyotetezeka komanso yosavuta. Chotulutsidwa chaulere cha nucleic acid chimakhala ndi zokolola zambiri, chiyero chokwanira, chokhazikika komanso chodalirika, ndipo makamaka choyenera kupangira malo ogwiritsira ntchito kwambiri.

Mphaka. Ayi Kukulitsa Kukula
4992413 2 ml × 50 ma preps
4992414 2 ml × 200 ma preps
4992917 2 ml × 1000 ma preps

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Chikwamacho chimakwaniritsa zofunikira pakuchotsa pamanja komanso kutulutsa kwa batch pamapulatifomu osiyanasiyana okwera kwambiri.
■ Zomwe zimapangidwa ndi zida zimakwaniritsa zofunikira pakuyesa kwakanthawi kotsika ndikuwunika kwa NGS.
■ Izi ndizoyenera zitsanzo za seramu ndi plasma zomwe zimakhala ndi 2-5 ml.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)


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    Experimental ExampleExperimental Example cfDNA idachotsedwa ndi TIANGEN Magnetic Serum / Plasma DNA Maxi Kit ndi chinthu chofunikira kuchokera kwa Supplier M kuchokera ku 2 ml zitsanzo za seramu.

    Chithunzi 1: Chitani kusanthula kwa Agilent2100 pambuyo pomanga laibulale ya cfDNA.

    Gulu 1: Qubit HS: Ndende ya DNA yomwe imadziwika ndi Qubit.

    Mtengo wa Ct: qPCR zotsatira zowonjezera ndi globulin primers.

    Agilent 2100: Kukhazikika pa 300 bp malo omanga laibulale ya DNA yoyesedwa ndi Agilent 2100 Chip.

    Q: Ndi DNA yaying'ono kapena ayi mu mlengalenga.

    A-1 Kuchuluka kwa maselo kapena kachilombo koyambitsa nyereredwe yoyamba - Limbikitsani kuchuluka kwa ma cell kapena ma virus.

    A-2 Kuperewera kwa sampuli -Zitsanzozo sizinasakanikirane bwino ndi chosungira cha lysis. Amalangizidwa kuti azisakanikirana bwino ndi kutentha kwa thupi maulendo 1-2. —Maselo osakwanira omwe amabwera chifukwa cha kuchepa kwa proteinase K. —Wosakwanira cell lysis kapena kuwonongeka kwa mapuloteni chifukwa chosakwanira nthawi yosamba. Amalangizidwa kuti azidula zidutswazo tizidutswa tating'ono ndikuwonjezera nthawi yosamba kuti achotse zotsala zonse mu lysate.

    A-3 Kusakwanira kwa DNA kutsatsa. - Palibe ethanol kapena peresenti yotsika m'malo mwa 100% ethanol yomwe idawonjezeredwa lysate isanatumizidwe ku gawo loyambira.

    A-4 Mtengo wa pH wa elution buffer ndiwotsika kwambiri. -Sinthani pH kuti ikhale pakati pa 8.0-8.3.

    Q: DNA siyimagwira bwino poyeserera koyesera kwa enzymatic reaction.

    Mowa wotsalira mumtambo.

    —Pali zotsalira zotsukira PW mumlengalenga. Mowa ungachotsedwe poyika gawo loyenda la 3-5 min, kenako ndikuyika kutentha kapena 50 ℃ incubator ya 1-2 min.

    Q: Kuwonongeka kwa DNA

    A-1 Chitsanzocho sichatsopano. —Tengani chitsanzo chabwino cha DNA kuti muwone ngati DNA yomwe ili mchitsanzocho yawonongeka.

    A-2 Chithandizo chisanachitike. —Kumachitika chifukwa cha utsi wambiri wa nayitrogeni, kupezanso chinyezi, kapena kuchuluka kwa chitsanzocho.

    Q: Momwe mungagwiritsire ntchito chithandizo choyambirira cha gDNA?

    Zoyesererazi ziyenera kusiyanasiyana pamitundu yosiyanasiyana. Pazitsamba, onetsetsani kuti mukugaya madzi asafe. Zitsanzo za nyama, homogenate kapena sungani bwino madzi asafe. Pazitsanzo zokhala ndi makoma osavuta kuswa, monga mabakiteriya a G + ndi yisiti, amalimbikitsidwa kugwiritsa ntchito lysozyme, lyticase kapena njira zamakina kuti athyole makoma am'maselo.

    Q: Kodi pali kusiyana kotani pakati pa zida zitatu zobzala gDNA 4992201/4992202, 4992724/4992725, 4992709/4992710?

    4992201/4992202 Plant Genomic DNA Kit imagwiritsa ntchito njira yozikika yomwe imafunikira chloroform kuti izichotse. Makamaka ndi oyenera mitundu yazomera zosiyanasiyana, komanso ufa wouma wouma. Hi-DNAsecure Plant Kit imapangidwanso m'mbali, koma osafunikira kuchotsedwa kwa phenol / chloroform, kuti ikhale yotetezeka komanso yopanda poizoni. Ndioyenera kumera ndi polysaccharides komanso polyphenol. 4992709/4992710 DNAquick Plant System imagwiritsa ntchito njira yopangira madzi. Kuchotsa kwa phenol / chloroform sikufunikanso. Njira yodziyeretsera ndiyosavuta komanso yachangu yopanda malire pazitsanzo zoyambira, kotero ogwiritsa ntchito amatha kusintha ndalamazo mosinthasintha malinga ndi zoyeserera. Kukula kwakukulu kwa zidutswa za gDNA kumatha kupezeka ndi zokolola zambiri.

    Kodi zokolola za gDNA kuchokera ku 1 ml ya magazi ndi TIANamp Blood DNA Kit ndi ziti?

    DNA ya genomic idatengedwa m'magulu osiyanasiyana amwazi ndi TIANamp Blood DNA Kit. Zotsatira zake ndi izi. Zotsatirazo zalembedwa ngati zolembedwera zokha, zotsatira zake zenizeni zimadalira momwe zitsanzo zilili.

    faq

    Q: Kodi 4992207/4992208 ndi 4992722/4992723 zingagwiritsidwe ntchito kutulutsa magazi a magazi?

    Kutulutsa magazi kwamagazi a DNA kumatha kuchitidwa pogwiritsa ntchito ma reagents omwe amapezeka mu zida ziwirizi posintha ndondomekoyi pamalangizo apadera otulutsa magazi a DNA. Kope lofewa la pulogalamu yamagazi yamagazi ya DNA limatha kuperekedwa mukapempha.

    Q: Mukamagwiritsa ntchito TIANamp Genomic DNA Kit, momwe mungaphwanye minofu yatsopano ndikuyimitsidwa kwama cell?

    Yimitsani zitsanzo zatsopano ndi 1 ml PBS, saline wamba kapena buff buff. Kwathunthu homogenize nyemba ndi homogenizer ndi kusonkhanitsa precipitate pansi pa chubu ndi centrifuging. Tayani supernatant, ndikuyambiranso kuyambiranso ndi 200 μl buffer GA. Kuyeretsa kwa DNA kungachitike potsatira malangizo.

    Q: Kodi mungasankhe bwanji zopangira DNA kuchokera ku plasma, seramu ndi zitsanzo zamadzimadzi amthupi?

    Poyeretsa gDNA mu plasma, seramu ndi madzi madzimadzi, TIANamp Micro DNA Kit ikulimbikitsidwa. Poyeretsa kachilombo ka HIV kuchokera ku zitsanzo za seramu / plasma, TIANamp Virus DNA / RNA Kit ikulimbikitsidwa. Poyeretsa bakiteriya gDNA kuchokera ku ma seramu ndi zitsanzo za plasma, TIANamp Bacteria DNA Kit ikulimbikitsidwa (lysozyme iyenera kuphatikizidwa ndi bakiteriya wabwino). Mwa zitsanzo za malovu, Hi-Swab DNA Kit ndi TIANamp Bacteria DNA Kit amalimbikitsidwa.

    Q: Kodi mungasankhe bwanji zida zogulira za gDNA pazitsanzo za bowa?

    Chida chodzikongoletsera cha DNAsec kapena DNAquick Plant System ndikulimbikitsidwa pakuchotsa mafangasi a genome. Pochotsa yisiti, TIANamp Yisiti ya Kit ikulimbikitsidwa (lyticase iyenera kudzikonzekeretsa).

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