TGuide Magazi a Genomic DNA Kit
Mapulogalamu
Genome yoyeretsedwa itha kugwiritsidwa ntchito mwachindunji mu PCR, RT-PCR, enzyme digestion reaction, Southern hybridization ndi zoyeserera zina.
Mawonekedwe
■ Kuchotsa kosavuta komanso kwachangu: Zogulitsa za TGuide zimadalira mfundo ya kuyeretsa kwa nucleic acid ndi mikanda yamaginito, ndipo kutulutsa kwa genomic DNA kumatha kumaliza mu 44 min.
■ Zotsatira zodalirika: DNA ya genomic yotulutsidwa ndi chidacho ilibe zodetsa monga RNA ndi protein, ndipo itha kugwiritsidwa ntchito mwachindunji ku PCR kapena fluorescence quantitative PCR.
■ Palibe phenol ndi chloroform yotulutsa: Palibe mankhwala osungunulira zinthu zoipa omwe angawononge thupi la munthu monga phenol ndi chloroform omwe amafunikira.
■ Kukula kocheperako koyambirira: 200 μl, 400 μl, 1.2 ml yamagazi athunthu kapena 1-3 ml ya leukocyte yomwe idalekanitsidwa kale imatha kutulutsidwa mwachindunji posankha chida chofanana.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
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Zotsatira za genomic DNA yotengedwa mu 200 μl magazi athunthu omwe amathandizidwa ndi ma anticoagulants osiyanasiyana |
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DNA idasungunuka mu 200 μl elution buffer. M: 1 kb makwerero 1-5 Kuzindikira kwa zoletsa ma anticoagulants osiyanasiyana. Cholinga cha jini: Cbl-b jini, 1.7 kb P: Kulamulira bwino |
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Zotsatira za genomic DNA yotengedwa mu 400 μl kapena 600 μl magazi athunthu |
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DNA idasungunuka mu 100 μl elution buffer Genomic DNA yotengedwa mu 400 μl kapena 600 μl magazi athunthu M: DL15000 Chikhomo Kutsegula voliyumu: 2 μl |
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Zotsatira za genomic DNA yotengedwa mu 1.2 ml magazi athunthu |
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DNA idasungunuka mu 300 μl elution buffer Genomic DNA yotengedwa kuchokera ku 1.2 ml magazi athunthu M: DL15000 Chikhomo; Kutsegula voliyumu: 2 μl |
A-1 Kuchuluka kwa maselo kapena kachilombo koyambitsa nyereredwe yoyamba - Limbikitsani kuchuluka kwa ma cell kapena ma virus.
A-2 Kuperewera kwa sampuli -Zitsanzozo sizinasakanikirane bwino ndi chosungira cha lysis. Amalangizidwa kuti azisakanikirana bwino ndi kutentha kwa thupi maulendo 1-2. —Maselo osakwanira omwe amabwera chifukwa cha kuchepa kwa proteinase K. —Wosakwanira cell lysis kapena kuwonongeka kwa mapuloteni chifukwa chosakwanira nthawi yosamba. Amalangizidwa kuti azidula zidutswazo tizidutswa tating'ono ndikuwonjezera nthawi yosamba kuti achotse zotsala zonse mu lysate.
A-3 Kusakwanira kwa DNA kutsatsa. - Palibe ethanol kapena peresenti yotsika m'malo mwa 100% ethanol yomwe idawonjezeredwa lysate isanatumizidwe ku gawo loyambira.
A-4 Mtengo wa pH wa elution buffer ndiwotsika kwambiri. -Sinthani pH kuti ikhale pakati pa 8.0-8.3.
Mowa wotsalira mumtambo.
—Pali zotsalira zotsukira PW mumlengalenga. Mowa ungachotsedwe poyika gawo loyenda la 3-5 min, kenako ndikuyika kutentha kapena 50 ℃ incubator ya 1-2 min.
A-1 Chitsanzocho sichatsopano. —Tengani chitsanzo chabwino cha DNA kuti muwone ngati DNA yomwe ili mchitsanzocho yawonongeka.
A-2 Chithandizo chisanachitike. —Kumachitika chifukwa cha utsi wambiri wa nayitrogeni, kupezanso chinyezi, kapena kuchuluka kwa chitsanzocho.
Zoyesererazi ziyenera kusiyanasiyana pamitundu yosiyanasiyana. Pazitsamba, onetsetsani kuti mukugaya madzi asafe. Zitsanzo za nyama, homogenate kapena sungani bwino madzi asafe. Pazitsanzo zokhala ndi makoma osavuta kuswa, monga mabakiteriya a G + ndi yisiti, amalimbikitsidwa kugwiritsa ntchito lysozyme, lyticase kapena njira zamakina kuti athyole makoma am'maselo.
4992201/4992202 Plant Genomic DNA Kit imagwiritsa ntchito njira yozikika yomwe imafunikira chloroform kuti izichotse. Makamaka ndi oyenera mitundu yazomera zosiyanasiyana, komanso ufa wouma wouma. Hi-DNAsecure Plant Kit imapangidwanso m'mbali, koma osafunikira kuchotsedwa kwa phenol / chloroform, kuti ikhale yotetezeka komanso yopanda poizoni. Ndioyenera kumera ndi polysaccharides komanso polyphenol. 4992709/4992710 DNAquick Plant System imagwiritsa ntchito njira yopangira madzi. Kuchotsa kwa phenol / chloroform sikufunikanso. Njira yodziyeretsera ndiyosavuta komanso yachangu yopanda malire pazitsanzo zoyambira, kotero ogwiritsa ntchito amatha kusintha ndalamazo mosinthasintha malinga ndi zoyeserera. Kukula kwakukulu kwa zidutswa za gDNA kumatha kupezeka ndi zokolola zambiri.
DNA ya genomic idatengedwa m'magulu osiyanasiyana amwazi ndi TIANamp Blood DNA Kit. Zotsatira zake ndi izi. Zotsatirazo zalembedwa ngati zolembedwera zokha, zotsatira zake zenizeni zimadalira momwe zitsanzo zilili.
Kutulutsa magazi kwamagazi a DNA kumatha kuchitidwa pogwiritsa ntchito ma reagents omwe amapezeka mu zida ziwirizi posintha ndondomekoyi pamalangizo apadera otulutsa magazi a DNA. Kope lofewa la pulogalamu yamagazi yamagazi ya DNA limatha kuperekedwa mukapempha.
Yimitsani zitsanzo zatsopano ndi 1 ml PBS, saline wamba kapena buff buff. Kwathunthu homogenize nyemba ndi homogenizer ndi kusonkhanitsa precipitate pansi pa chubu ndi centrifuging. Tayani supernatant, ndikuyambiranso kuyambiranso ndi 200 μl buffer GA. Kuyeretsa kwa DNA kungachitike potsatira malangizo.
Poyeretsa gDNA mu plasma, seramu ndi madzi madzimadzi, TIANamp Micro DNA Kit ikulimbikitsidwa. Poyeretsa kachilombo ka HIV kuchokera ku zitsanzo za seramu / plasma, TIANamp Virus DNA / RNA Kit ikulimbikitsidwa. Poyeretsa bakiteriya gDNA kuchokera ku ma seramu ndi zitsanzo za plasma, TIANamp Bacteria DNA Kit ikulimbikitsidwa (lysozyme iyenera kuphatikizidwa ndi bakiteriya wabwino). Mwa zitsanzo za malovu, Hi-Swab DNA Kit ndi TIANamp Bacteria DNA Kit amalimbikitsidwa.
Chida chodzikongoletsera cha DNAsec kapena DNAquick Plant System ndikulimbikitsidwa pakuchotsa mafangasi a genome. Pochotsa yisiti, TIANamp Yisiti ya Kit ikulimbikitsidwa (lyticase iyenera kudzikonzekeretsa).
Zamgululi siyana
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