TIANamp FFPE DNA zida
Mawonekedwe
■ Kuyera kwambiri: Khungu la silika limachotsa zodetsa zambiri.
■ Kugwiritsa ntchito kwambiri: Oyenera gawo la parafini, chipika cha parafini, ndi zitsanzo zophatikizidwa ndi formalin.
■ Kuchita zinthu mwachangu: DNA imatha kupezeka mkati mwa ola limodzi.
Mfundo
Type: Spin column based
Zitsanzo: Gawo la parafini, chipika cha parafini, ndi zitsanzo zophatikizidwa ndi formalin
Cholowera: Genomic DNA
Kuyambitsa voliyumu: Zidutswa 5-8 zama parafini kapena minofu ya 30 mg mu parafini kapena formalin
Nthawi yogwiritsira ntchito: ~ 1 ora
Mapulogalamu otsika: PCR, SNP genotype kusanthula, kusanthula kwa STR, kusanthula kwa pharmacogenomics, kapangidwe ka laibulale ya NGS, ndi zina zambiri.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
DNA ya genomic yotulutsidwa ndi TIANamp FFPE DNA Kit ndi zinthu zofunikira kuchokera ku Supplier A zidakulitsidwa kukhala 190 bp, 330 bp, 500 bp, 650 bp ndi 1300 bp zidutswa. Zotsatira zake zikuwonetsa kuti DNA yotulutsidwa ndi TIANamp FFPE DNA Kit ili ndi zokolola zochuluka, zoyera kwambiri makamaka pazidutswa zazikulu.
Kuyamba zakuthupi: Mbewa ya chiwindi FFPE zitsanzo (zidutswa 5, 10 μm / chidutswa)
Ndondomeko: Tsatirani malangizo a chinthu chilichonse.
Kutsitsa kwa gel: gDNA idasangalatsidwa ndi 50 μl elution buffer ndipo idakwezedwa kukhala 190 bp, 330 bp 500 bp, 650 bp ndi 1300 bp. 5 μl ya 20 μl Zogulitsa za PCR zidakwezedwa pamsewu uliwonse pa 1% agarose gel. Electrophoresis inkachitika pa 6 V / cm kwa mphindi 20.
M: Chizindikiro cha TIANGEN D2000;
Kufotokozera: T: TIANamp FFPE DNA Kit;
Yankho: Choyenera kuchokera kwa Wogulitsa A.
A-1 Kuchuluka kwa maselo kapena kachilombo koyambitsa nyereredwe -Pinditsani kuchuluka kwa ma cell kapena ma virus.
A-2 Kuperewera kwa sampuli -Zitsanzozo sizinasakanikirane bwino ndi chosungira cha lysis. Amalangizidwa kuti azisakanikirana bwino ndi kutentha kwa thupi maulendo 1-2. —Maselo osakwanira omwe amabwera chifukwa cha kuchepa kwa proteinase K. —Wosakwanira cell lysis kapena kuwonongeka kwa mapuloteni chifukwa chosakwanira nthawi yosamba. Amalangizidwa kuti azidula zidutswazo tizidutswa tating'ono ndikuwonjezera nthawi yosamba kuti achotse zotsala zonse mu lysate.
A-3 Kusakwanira kwa DNA kutsatsa. - Palibe ethanol kapena peresenti yotsika m'malo mwa 100% ethanol yomwe idawonjezeredwa lysate isanatumizidwe ku gawo loyambira.
A-4 Mtengo wa pH wa elution buffer ndiwotsika kwambiri. -Sinthani pH kuti ikhale pakati pa 8.0-8.3.
Mowa wotsalira mumtambo.
—Pali zotsalira zotsukira PW mumlengalenga. Ethanol imatha kuchotsedwa poika pakati gawo la spin kwa 3-5 min, kenako ndikuyika firiji kapena 50 ℃ incubator ya 1-2 min.
A-1 Chitsanzocho sichatsopano. —Tengani chitsanzo chabwino cha DNA kuti muwone ngati DNA yomwe ili mchitsanzocho yawonongeka.
A-2 Chithandizo chisanachitike. -Kumachitika chifukwa chopera kwambiri nayitrogeni wamadzi, kupezanso chinyezi, kapena kuchuluka kwachitsanzo.
Zoyesererazi ziyenera kusiyanasiyana pamitundu yosiyanasiyana. Pazitsamba, onetsetsani kuti mukugaya madzi asafe. Zitsanzo za nyama, homogenate kapena sungani bwino madzi asafe. Pazitsanzo zokhala ndi makoma osavuta kuswa, monga mabakiteriya a G + ndi yisiti, amalimbikitsidwa kugwiritsa ntchito lysozyme, lyticase kapena njira zamakina kuti athyole makoma am'maselo.
4992201/4992202 Plant Genomic DNA Kit imagwiritsa ntchito njira yozikika yomwe imafunikira chloroform kuti izichotse. Makamaka ndi oyenera mitundu yazomera zosiyanasiyana, komanso ufa wouma wouma. Hi-DNAsecure Plant Kit imapangidwanso m'mbali, koma osafunikira kuchotsedwa kwa phenol / chloroform, kuti ikhale yotetezeka komanso yopanda poizoni. Ndioyenera kumera ndi polysaccharides komanso polyphenol. 4992709/4992710 DNAquick Plant System imagwiritsa ntchito njira yopangira madzi. Kuchotsa kwa phenol / chloroform sikufunikanso. Njira yodziyeretsera ndiyosavuta komanso yachangu yopanda malire pazitsanzo zoyambira, kotero ogwiritsa ntchito amatha kusintha ndalamazo mosinthasintha malinga ndi zoyeserera. Kukula kwakukulu kwa zidutswa za gDNA kumatha kupezeka ndi zokolola zambiri.
DNA ya genomic idatengedwa m'magulu osiyanasiyana amwazi ndi TIANamp Blood DNA Kit. Zotsatira zake ndi izi. Zotsatirazo zalembedwa ngati zolembedwera zokha, zotsatira zake zenizeni zimadalira momwe zitsanzo zilili.
Kutulutsa magazi kwamagazi a DNA kumatha kuchitidwa pogwiritsa ntchito ma reagents omwe amapezeka mu zida ziwirizi posintha ndondomekoyi pamalangizo apadera otulutsa magazi a DNA. Kope lofewa la pulogalamu yamagazi yamagazi ya DNA limatha kuperekedwa mukapempha.
Yimitsani zitsanzo zatsopano ndi 1 ml PBS, saline wamba kapena buff buff. Kwathunthu homogenize nyemba ndi homogenizer ndi kusonkhanitsa precipitate pansi pa chubu ndi centrifuging. Tayani supernatant, ndikuyambiranso kuyambiranso ndi 200 μl buffer GA. Kuyeretsa kwa DNA kungachitike potsatira malangizo.
Poyeretsa gDNA mu plasma, seramu ndi madzi madzimadzi, TIANamp Micro DNA Kit ikulimbikitsidwa. Poyeretsa kachilombo ka HIV kuchokera ku zitsanzo za seramu / plasma, TIANamp Virus DNA / RNA Kit ikulimbikitsidwa. Poyeretsa bakiteriya gDNA kuchokera ku ma seramu ndi zitsanzo za plasma, TIANamp Bacteria DNA Kit ikulimbikitsidwa (lysozyme iyenera kuphatikizidwa ndi bakiteriya wabwino). Mwa zitsanzo za malovu, Hi-Swab DNA Kit ndi TIANamp Bacteria DNA Kit amalimbikitsidwa.
Chida chodzikongoletsera cha DNAsec kapena DNAquick Plant System ndikulimbikitsidwa pakuchotsa mafangasi a genome. Pochotsa yisiti, TIANamp Yisiti ya Kit ikulimbikitsidwa (lyticase iyenera kudzikonzekeretsa).
Zamgululi siyana
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