TIANprep N96 Plasmid zida

Kuwunika nthawi yeniyeni ya lysis komanso kutulutsa kwapamwamba kwambiri kwa plasmids oyera kwambiri

Kakhungu ka N96 Plate CP3 mu chida ichi kamakhala ndi nkhani ya silica matrix mwapadera yopangidwa ndi TIANGEN, yomwe imatha kutsatsa DNA ya plasmid moyenera, kuchotsa mapuloteni ndi zonyansa zina zonse, motero kuwonetsetsa kuti plasmid yochotsedwa. Mpaka 1.3 ml ya mabakiteriya amtundu wa plasmid amatha kukonzedwa pachitsime chilichonse, pomwe mpaka 5-10 μg ya plasmid DNA itha kuchotsedwa mwachangu (zokololazo ndizogwirizana ndi zinthu monga nambala ya plasmid, boma la bakiteriya, ndi zina zambiri. ). Plasmid DNA yotulutsidwa ndi zida zitha kugwiritsidwa ntchito poyesa ma biology ofufuza monga kuletsa kugaya kwa enzyme, kusanja, kuwunikira laibulale, ligation ndi kusintha.

Mphaka. Ayi	Kukulitsa Kukula
4992892	4 ma preps
4992893	24 preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Kuthamanga mwachangu.
■ Pokhala ndi reagent yapadera ya TIANRed, imatha kuzindikira bwino kuchuluka kwa lysis ya nyembayi pakuwotchera kuti zitsimikizire kuti plasmid DNA ndiyabwino kwambiri.
■ Kuyera kwambiri: Plasmid DNA yoyeretsedwa itha kugwiritsidwa ntchito mwachindunji m'mayeso am'munsi.
■ Chida ichi chitha kugwiritsidwa ntchito m'malo opangira zida zamagetsi zamagetsi.

TIANRed Mtundu chizindikiro

TIANRed Color indicator:

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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Ena: TIANamp Magazi a DNA Midi Kit

	Plasmid DNA idachotsedwa mu 1 ml ya msuzi wa Escherichia coli (TOP10) wamtsogolo (LB sing'anga) pogwiritsa ntchito TIANprep Highpure N96 Plasmid Kit, ndipo pafupifupi 5-6 μg wa chiyero chokwanira cha pBS plasmid chidapezeka. Plasmid idalowetsedwa pachitsime chilichonse cha 1% ya agarose gel, ndipo imapezeka ndi electrophoresis
	Plasmid DNA idachotsedwa mu 1 ml ya msuzi wa Escherichia coli (TOP10) wamtsogolo (LB sing'anga) pogwiritsa ntchito TIANprep Highpure N96 Plasmid Kit, ndipo pafupifupi 5-6 μg wa chiyero chokwanira cha pBS plasmid chidapezeka. Vector: pBS; Kupsyinjika kwa Plasmid: TOP10. Plasmid idayendetsedwa ndi ABI. Ndondomeko ya PRISM 3730XL DNA.