■ Zizindikiro zamtundu zimawonjezeredwa kwa ma reagents kuti apewe zolakwika zowonjezera kapena kusiya chifukwa cha kuchuluka kwa zitsanzo.
■ Mtundu wa SuperReal PreMix uli ndi phindu lowala kwambiri la fluorescence, chidwi chachikulu komanso kudziwika kwapadera.
■ Yoyenera ma probes ambiri wamba a fulorosenti (FAM, HEX, NED, etc.). Chizindikirocho sichingakhudze kuwala kwa kafukufuku.
■ Zoyenera pazida zosiyanasiyana za PCR (Roche, Bio-rad, ABI, ndi zina zambiri).
Ndioyenera mitundu ingapo ya zoyeserera monga kusanthula kwamawu ndi kuzindikira kwa acidic pogwiritsa ntchito njira zofufuzira pazida zenizeni zenizeni za PCR. Ndizoyenera makamaka pakuyesa kwapamwamba kwambiri ndi zitsanzo zambiri ndi kutsitsa kovuta.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
Chithunzi 1. Zithunzi zama curve zokulitsa ndi ma curve oyenera omwe amapezeka potengera mtundu wa anthu HSUBC pogwiritsa ntchito TIANGEN SuperReal PreMix Plus (Probe) (A) ndi SuperReal PreMix Colour (Probe) (B). Chithunzicho chimasungunuka kwama gradients 5 ozungulira. Makhalidwe a Ct (C) akuwonetsa kuti kukulitsa kwa zida ziwirizi kumvetsetsa komanso kukhazikika. Chida chomwe chimagwiritsidwa ntchito ndi ABI 7500 ndipo gulu lofufuzira la fluorescent ndi FAM.
Chiyambireni kukhazikitsidwa, fakitole yathu yakhala ikupanga zinthu zoyambirira motsatira mfundoyo
yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..