■ Zinthu zosungira: Chikwama ichi chimatha kusungidwa kwa sabata limodzi kutentha, tsiku limodzi pa 37 ℃, komanso mwezi umodzi pa 4 ℃. Zitsanzo za minofu, kumiza 4 ℃ usiku umodzi, ndikusamutsa -20 ℃ kapena -80 ℃ kuti musunge nthawi yayitali.
■ Kuzizira mobwerezabwereza:
■ Kugwiritsa ntchito kumunsi: Mukachotsa zitsanzo kuchokera ku RNAstore Reagent, RNA yathunthu imatha kutulutsidwa ndi TIANGEN's TRNzol, RNAprep Pure, RNAsimple reagents and kits.
■ RNAstore imangoyenera zitsanzo za minofu yatsopano.
■ RNAstore siyabwino pazitsanzo za minofu yazomera.
■ Kuchuluka kwa kuchuluka kwa minofu ndi RNAstore Reagent kuyenera kukhala osachepera 1: 10 (mwachitsanzo, 100 mg minofu, pakufunika 1 ml ya RNAstore).
■ Kukula kwa mbali iliyonse yachitsanzo sikuyenera kupitirira masentimita 0,5 kuti zitsimikizire kuti RNAstore imatha kulowa mwachangu.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
Zakuthupi: 15 mg Rat minofu ya chiwindi Njira: 0,5 g makoswe a chiwindi (osungidwa mu RNAstore Reagent) amasungidwa ku 37 ℃, kutentha kwapakati ndi 4 ℃ motsatana. RNA yonse kuchokera ku 15 mg makoswe amtundu wa chiwindi omwe amasungidwa kutentha kosiyanasiyana adadzipatula pogwiritsa ntchito TRNzol Reagent (Cat. No. 4992730). Zotsatira: Chonde onani chithunzi cha agarose gel electrophoresis pamwambapa. 2-4 μl of 100 μl eluates adakwezedwa pamsewu. C (kuwongolera koyenera): zitsanzo za minofu zomwe zasungidwa -80 ℃. Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose. |
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Zakuthupi: 15 mg Rat minofu ya chiwindi Njira: 0,5 g makoswe amtundu wa chiwindi (omwe amasungidwa mu RNAstore Reagent) adawundidwa nthawi 5, 10, 15 ndi 20 motsatana. RNA yonse yochokera ku 15 mg makoswe a ziwindi zamtundu wa chiwindi chomwe chimaundana nthawi zosiyanasiyana adadzipatula pogwiritsa ntchito TRNzol Reagent (Cat. No. 4992730). Zotsatira: Chonde onani chithunzi cha agarose gel electrophoresis pamwambapa. 2-4 μl of 100 μl eluates adakwezedwa pamsewu. C (kuwongolera koyenera): zitsanzo za minofu zomwe zasungidwa -80 ℃. 5, 10, 15, 20: nthawi zowundana kwambiri za zitsanzo. Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose. |
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yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..