RNAsimple Chiwerengero cha RNA Kit

Pazogwiritsira ntchito RNA yokwanira bwino pogwiritsa ntchito gawo logwiritsidwa ntchito kwambiri la centrifugal.

RNAsimple Total RNA Kit imagwiritsa ntchito njira yatsopano yochotsera RNA potengera njira ya guanidinium isothiocyanate / phenol. Buffer RZ yopangidwa mwapadera ndi TIANGEN imatha kuchotsa ma genomic DNA ndi mapuloteni m'maselo mwachangu komanso moyenera, ndikupangitsa kuti RNA yopezedwa ikhale yoyera komanso yolimba.
Chida ichi chimagwiritsidwa ntchito kulekanitsa RNA yathunthu ndi magazi, maselo a nyama, ziphuphu, ndi zotupa. Mzere uliwonse wa spin umatha kupanga 50-100 mg minofu kapena 5 × 10⁶maselo nthawi imodzi ndipo amatha kuthana ndi mitundu yambiri nthawi imodzi. Zomwe zimachitikazo zimatha kumaliza ola limodzi, ndipo RNA yonse yotulutsidwa imakhala ndi zokolola zochuluka, zoyera bwino, zopanda DNA komanso kuipitsidwa kwa mapuloteni, ndipo itha kugwiritsidwa ntchito m'mayeso osiyanasiyana akumunsi.

Mphaka. Ayi	Kukulitsa Kukula
4992858	50 preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Kayendedwe

Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Kuyera kwa RNA kogwiritsa ntchito kwambiri ndi koyenera kugwiritsira ntchito mosavutikira.
■ Ntchito zazikulu: RNA yoyeretsedwa itha kugwiritsidwa ntchito pazitsanzo zosiyanasiyana zoyesera.
■ Kuyesaku kumatha kumaliza mu ola limodzi pogwiritsa ntchito zosavuta.

Mapulogalamu

■ RT-PCR
■ Northern Blot, Dot Blot.
■ PCR Yeniyeni
■ Kuwunika kwa PolyA, kumasulira kwa ma vitro, kusanthula kwa RNase chitetezo, kupangika kwa ma molekyulu.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

Previous: Zamgululi RNAclean zida

Ena: RNAprep Koyera yaying'ono zida

Zakuthupi: 20 mg rat spleen minofu
Njira: RNA yathunthu yamatenda amphako idadzipatula pogwiritsa ntchito RNAsimple Total RNA Kit.
Zotsatira: Chonde onani chithunzi cha agarose gel electrophoresis pamwambapa. 2-4 μl of 100 μl eluates adakwezedwa pamsewu. Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose.

Q: Kutsekeka kwamagawo

A-1 Cell lysis kapena homogenization sikokwanira

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa kuchuluka kwa nyemba zomwe mukugwiritsa ntchito kapena kuonjezera kuchuluka kwa lysis buffer.

Q: Zokolola zochepa za RNA

A-1 Kusakwanira kwa cell lysis kapena homogenization

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

- Chonde onani kuthekera kwakukulu kokonza.

A-3 RNA siyosankhidwa kwathunthu kuchokera pagawo

---- Mukatha kuwonjezera madzi opanda RNase, siyani kwa mphindi zochepa centrifuging.

A-4 Ethanol mumlengalenga

- Pambuyo kutsukidwa, centrifuge kachiwiri ndikuchotsa chotsuka chotsuka momwe zingathere.

A-5 Cell chikhalidwe sing'anga sichichotsedweratu

- Mukamasonkhanitsa maselo, chonde onetsetsani kuti muchotse sing'anga momwe mungathere.

A-6 Maselo osungidwa mu RNAstore sakhala otsekemera kwenikweni

---- RNAstore osalimba ndi wamkulu kuposa sing'anga wamba wamaselo; kotero mphamvu ya centrifugal iyenera kukulitsidwa. Akuti centrifuge pa 3000x g.

Zolemba za A-7 Low RNA komanso kuchuluka kwake mchitsanzo

- Gwiritsani ntchito chitsanzo chabwino kuti muwone ngati zokolola zochepa zimayambitsidwa ndi chitsanzocho.

Q: Kuwonongeka kwa RNA

A-1 Nkhaniyi siyatsopano

- Minyewa yatsopano imayenera kusungidwa mu nayitrogeni wamadzi nthawi yomweyo kapena kuyiyika mu reagent ya RNAstore kuti zitsimikizidwe kuti zimachokera.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-3 RNase kuipitsidwan

- Ngakhale gawo lotetezedwa mulibe RNase, ndikosavuta kuipitsa RNase panthawi yazochulukirapo ndipo iyenera kusamalidwa.

Kuwononga kwa A-4 Electrophoresis

---- Sinthanitsani cholumikizira cha electrophoresis ndikuwonetsetsa kuti zofunikira ndi Kutsegula Buffer zilibe vuto la RNase.

A-5 Kutsitsa kwambiri kwa electrophoresis

---- Kuchepetsa kuchuluka kwa potsegula, kutsitsa kwa chitsime chilichonse sikuyenera kupitirira 2 μg.

Q: kuipitsidwa kwa DNA

A-1 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-2 Zitsanzo zina zimakhala ndi DNA yambiri ndipo amatha kuchiritsidwa ndi DNase.

- Chitani chithandizo cha RNase-Free DNase ku yankho la RNA, ndipo RNA itha kugwiritsidwa ntchito mwachindunji poyeserera pambuyo pothandizidwa, kapena itha kuyeretsedwa ndi zida zoyeserera za RNA.

Q: Kodi mungachotse bwanji RNase pazomwe mungagwiritse ntchito poyesera ndi magalasi?

Zamagalasi, zophikidwa pa 150 ° C kwa 4 h. Pazitsulo zapulasitiki, zomizidwa mu 0,5 M NaOH kwa mphindi 10, kenako kutsukidwa bwino ndi madzi opanda RNase kenako ndikuwotcha kuti muchotse RNase. Ma reagents kapena mayankho omwe amagwiritsidwa ntchito poyeserera, makamaka madzi, ayenera kukhala opanda RNase. Gwiritsani ntchito madzi opanda RNase pamakonzedwe onse a reagent (onjezerani madzi mu botolo loyera lagalasi, onjezerani DEPC pamlingo womaliza wa 0,1% (V / V), gwedezani usiku umodzi ndi autoclave).

Lembani uthenga wanu apa ndi kutumiza kwa ife