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RNAprep Chomera Choyera Chowonjezera

Kuyeretsa RNA yonse kuchokera ku polysaccharides & zitsanzo zolemera za polyphenolics.

RNAprep Pure Plant Plus Kit (Polysaccharides & Polyphenolics-Wolemera) ndi kachipangizo kakang'ono koyeretsa kachitsulo ka RNA kamene kamapangidwira mitundu yambiri ya zomera ndi polysaccharides ndi polyphenolics. Amaperekedwa ndi Buffer SL yokhala ndi luso labwino kwambiri la lysis. RNA yoyera kwambiri yopanda gDNA imatha kutulutsidwa ku nthochi, mavwende, maapulo, mapeyala, ma tubers a mbatata, mbatata, masamba a thonje, rose, nyemba, mpunga ndi singano zoyera pasanathe ola limodzi .

Mphaka. Ayi	Kukulitsa Kukula
4992239	50 preps

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Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Zoyang'aniridwa: Zimapangidwa mwapadera ngati zitsanzo zazomera zomwe zimakhala zovuta kuzitulutsa, monga polysaccharides ndi michere yolemera polyphenolics. Njirayi imakonzedweratu, ndipo zotsatira zake ndizodalirika.
■ Kuchotsa kwa gDNA koyenera: DNase I yodalirika kwambiri imaperekedwa kuti ichotse gDNA mwachangu.
■ Mosavuta komanso mwachangu: Kuyeserera kwa RNA kumatha kumaliza ola limodzi.
■ Poizoni wotetezedwa komanso wotsika: palibe mankhwala ofunikira a reagents monga phenol ndi chloroform omwe amafunikira.

Mapulogalamu

Chida ichi chitha kugwiritsidwa ntchito mwachindunji pama kuyesa osiyanasiyana a biology monga RT-PCR, Real-time PCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, and cloning, etc.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

Previous: Zamgululi RNALock Wokonda

Ena: RNAprep Koyera Hi-Magazi Kit

RNA yonse idatengedwa kuchokera ku 100 mg nyama zamtchire, chivwende, apulo ndi peyala, ma tubers a mbatata ndi mbatata, masamba a thonje, rosi, nyemba, mpunga ndi singano zoyera motsatana pogwiritsa ntchito RNAprep Pure Plant Plus Kit. 4-6 μl ya 30 μl eluates adakwezedwa pamsewu.
M: TIANGEN Chizindikiro III;
Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose.
Zotsatira: RNAprep Pure Plant Plus Kit imatha kuchotsa kuyera kwambiri, zokolola zambiri komanso kukhulupirika kwathunthu ku RNA kuchokera kuzitsanzo za polyshencharides & polyphenolics.

Q: Kutsekeka kwamagawo

A-1 Cell lysis kapena homogenization sikokwanira

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa kuchuluka kwa nyemba zomwe mukugwiritsa ntchito kapena kuonjezera kuchuluka kwa lysis buffer.

Q: Zokolola zochepa za RNA

A-1 Kusakwanira kwa cell lysis kapena homogenization

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

- Chonde onani kuthekera kwakukulu kokonza.

A-3 RNA siyosankhidwa kwathunthu kuchokera pagawo

---- Mukatha kuwonjezera madzi opanda RNase, siyani kwa mphindi zochepa centrifuging.

A-4 Ethanol mumlengalenga

- Pambuyo kutsukidwa, centrifuge kachiwiri ndikuchotsa chotsuka chotsuka momwe zingathere.

A-5 Cell chikhalidwe sing'anga sichichotsedweratu

- Mukamasonkhanitsa maselo, chonde onetsetsani kuti muchotse sing'anga momwe mungathere.

A-6 Maselo osungidwa mu RNAstore sakhala otsekemera kwenikweni

---- RNAstore osalimba ndi wamkulu kuposa sing'anga wamba wamaselo; kotero mphamvu ya centrifugal iyenera kukulitsidwa. Akuti centrifuge pa 3000x g.

Zolemba za A-7 Low RNA komanso kuchuluka kwake mchitsanzo

- Gwiritsani ntchito chitsanzo chabwino kuti muwone ngati zokolola zochepa zimayambitsidwa ndi chitsanzocho.

Q: Kuwonongeka kwa RNA

A-1 Nkhaniyi siyatsopano

- Minyewa yatsopano imayenera kusungidwa mu nayitrogeni wamadzi nthawi yomweyo kapena kuyiyika mu reagent ya RNAstore kuti zitsimikizidwe kuti zimachokera.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-3 RNase kuipitsidwan

- Ngakhale gawo lotetezedwa mulibe RNase, ndikosavuta kuipitsa RNase panthawi yazochulukirapo ndipo iyenera kusamalidwa.

Kuwononga kwa A-4 Electrophoresis

---- Sinthanitsani cholumikizira cha electrophoresis ndikuwonetsetsa kuti zofunikira ndi Kutsegula Buffer zilibe vuto la RNase.

A-5 Kutsitsa kwambiri kwa electrophoresis

---- Kuchepetsa kuchuluka kwa potsegula, kutsitsa kwa chitsime chilichonse sikuyenera kupitirira 2 μg.

Q: kuipitsidwa kwa DNA

A-1 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-2 Zitsanzo zina zimakhala ndi DNA yambiri ndipo amatha kuchiritsidwa ndi DNase.

- Chitani chithandizo cha RNase-Free DNase ku yankho la RNA, ndipo RNA itha kugwiritsidwa ntchito mwachindunji poyeserera pambuyo pothandizidwa, kapena itha kuyeretsedwa ndi zida zoyeserera za RNA.

Q: Kodi mungachotse bwanji RNase pazomwe mungagwiritse ntchito poyesera ndi magalasi?

Zamagalasi, zophikidwa pa 150 ° C kwa 4 h. Pazitsulo zapulasitiki, zomizidwa mu 0,5 M NaOH kwa mphindi 10, kenako kutsukidwa bwino ndi madzi opanda RNase kenako ndikuwotcha kuti muchotse RNase. Ma reagents kapena mayankho omwe amagwiritsidwa ntchito poyeserera, makamaka madzi, ayenera kukhala opanda RNase. Gwiritsani ntchito madzi opanda RNase pamakonzedwe onse a reagent (onjezerani madzi mu botolo loyera lagalasi, onjezerani DEPC pamlingo womaliza wa 0,1% (V / V), gwedezani usiku umodzi ndi autoclave).

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