Chida Choyera cha RNAprep Kit

Kuyeretsa mpaka 100 μg RNA yathunthu kumatenda a nyama.

RNAprep Pure Tissue Kit imapereka njira yachangu, yosavuta, komanso yotsika mtengo yoyeretsera RNA yathunthu kumatenda a nyama pogwiritsa ntchito gawo loyenda bwino komanso njira yapadera yolumikizira. Chikwamacho chimaphatikizapo ma RNase-Free spin mizati CR3 yoyeretsera RNA yapamwamba kwambiri pogwiritsa ntchito ukadaulo wa silika-membrane. Mtengo wapamwamba kwambiri wa RNA ukhoza kupezeka mumphindi 40-50 mosadetsedwa komanso ulibe kuwonongeka kwa protein ndi genomic DNA.

Mphaka. Ayi	Kukulitsa Kukula
4992236	50 preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Zogwiritsira ntchito zolimba za nyama zimapangitsa kuti njirayi ikhale yosavuta komanso yosavuta.
■ DNase Wapadera I amachepetsa kuipitsidwa kwa DNA.
■ Kuyera kwapamwamba komanso kugwiritsa ntchito RNA ndikoyenera kugwiritsira ntchito mosavutikira.
■ Palibe chochotsa phenol / chloroform, palibe LiCl ndi mpweya wa ethanol, ndipo palibe CsCl gradients centrifugation yomwe ikufunika, zomwe zimapangitsa kuti ntchitoyi ikhale yotetezeka komanso yodalirika.

Mapulogalamu

■ RT-PCR.
■ Northern Blot, Dot Blot.
■ PCR Yeniyeni.
■ Kusanthula kwa Chip.
■ Kuwunika kwa PolyA, kumasulira kwa ma vitro, kusanthula kwa RNase ndi kupangika kwa ma molekyulu.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

Previous: Zamgululi RNAprep Koyera Cell / Bacteria Kit

Ena: RNAprep Koyera FFPE zida

Zakuthupi: 20 mg embryo (masiku 13), 15 mg impso, 10 mg chiwindi, 15 mg ndulu, 10 mg thymus, 20 mg lung
Njira: RNA yonse yamitundu yosiyanasiyana yamakoswe idatsukidwa pogwiritsa ntchito RNAprep Pure Tissue Kit.
Zotsatira: Chithunzi cha agarose gel electrophoresis chikuwonetsedwa pamwambapa. 2-4 μl of 100 μl eluates adakwezedwa pamsewu.
M: TIANGEN DNA Chizindikiro III;
Njira 1-2: Mwana wosabadwa (masiku 13); Njira 3: Impso; Msewu 4-6: Chiwindi; Njira 7: Nthata; Njira 8: Thymus; Njira 9: Mapapu.
Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose gel ..

Q: Kutsekeka kwamagawo

A-1 Cell lysis kapena homogenization sikokwanira

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa kuchuluka kwa nyemba zomwe mukugwiritsa ntchito kapena kuonjezera kuchuluka kwa lysis buffer.

Q: Zokolola zochepa za RNA

A-1 Kusakwanira kwa cell lysis kapena homogenization

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

- Chonde onani kuthekera kwakukulu kokonza.

A-3 RNA siyosankhidwa kwathunthu kuchokera pagawo

---- Mukatha kuwonjezera madzi opanda RNase, siyani kwa mphindi zochepa centrifuging.

A-4 Ethanol mumlengalenga

- Pambuyo kutsukidwa, centrifuge kachiwiri ndikuchotsa chotsuka chotsuka momwe zingathere.

A-5 Cell chikhalidwe sing'anga sichichotsedweratu

- Mukamasonkhanitsa maselo, chonde onetsetsani kuti muchotse sing'anga momwe mungathere.

A-6 Maselo osungidwa mu RNAstore sakhala otsekemera kwenikweni

---- RNAstore osalimba ndi wamkulu kuposa sing'anga wamba wamaselo; kotero mphamvu ya centrifugal iyenera kukulitsidwa. Akuti centrifuge pa 3000x g.

Zolemba za A-7 Low RNA komanso kuchuluka kwake mchitsanzo

- Gwiritsani ntchito chitsanzo chabwino kuti muwone ngati zokolola zochepa zimayambitsidwa ndi chitsanzocho.

Q: Kuwonongeka kwa RNA

A-1 Nkhaniyi siyatsopano

- Minyewa yatsopano imayenera kusungidwa mu nayitrogeni wamadzi nthawi yomweyo kapena kuyiyika mu reagent ya RNAstore kuti zitsimikizidwe kuti zimachokera.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-3 RNase kuipitsidwan

- Ngakhale gawo lotetezedwa mulibe RNase, ndikosavuta kuipitsa RNase panthawi yazochulukirapo ndipo iyenera kusamalidwa.

Kuwononga kwa A-4 Electrophoresis

---- Sinthanitsani cholumikizira cha electrophoresis ndikuwonetsetsa kuti zofunikira ndi Kutsegula Buffer zilibe vuto la RNase.

A-5 Kutsitsa kwambiri kwa electrophoresis

---- Kuchepetsa kuchuluka kwa potsegula, kutsitsa kwa chitsime chilichonse sikuyenera kupitirira 2 μg.

Q: kuipitsidwa kwa DNA

A-1 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-2 Zitsanzo zina zimakhala ndi DNA yambiri ndipo amatha kuchiritsidwa ndi DNase.

- Chitani chithandizo cha RNase-Free DNase ku yankho la RNA, ndipo RNA itha kugwiritsidwa ntchito mwachindunji poyeserera pambuyo pothandizidwa, kapena itha kuyeretsedwa ndi zida zoyeserera za RNA.

Q: Kodi mungachotse bwanji RNase pazomwe mungagwiritse ntchito poyesera ndi magalasi?

Zamagalasi, zophikidwa pa 150 ° C kwa 4 h. Pazitsulo zapulasitiki, zomizidwa mu 0,5 M NaOH kwa mphindi 10, kenako kutsukidwa bwino ndi madzi opanda RNase kenako ndikuwotcha kuti muchotse RNase. Ma reagents kapena mayankho omwe amagwiritsidwa ntchito poyeserera, makamaka madzi, ayenera kukhala opanda RNase. Gwiritsani ntchito madzi opanda RNase pamakonzedwe onse a reagent (onjezerani madzi mu botolo loyera lagalasi, onjezerani DEPC pamlingo womaliza wa 0,1% (V / V), gwedezani usiku umodzi ndi autoclave).

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