RNAprep Koyera Chomera zida

Poyeretsa RNA yonse kuchokera ku zomera ndi bowa.

RNAprep Pure Plant Kit imapereka njira yachangu, yosavuta, komanso yotsika mtengo yoyeretsera RNA yathunthu pazitsanzo zazomera pogwiritsa ntchito gawo loyenda bwino komanso njira yapaderadera. Chikwamacho chimaphatikizapo RNase-Free Filtration Column CS yopangira ma homogenizing ndi kusefa viscous chomera kapena ma lysates a fungal, ndikuzungulira gawo CR3 yoyeretsa RNA yapamwamba kwambiri pogwiritsa ntchito ukadaulo wa silika-membrane. Makhalidwe apamwamba a RNA amatha kupezeka mumphindi 30-40. Njira yonseyi ndiyosavuta, yosavuta komanso yotetezeka kuti igwire ntchito ndi poizoni wotsika. RNA yomwe idalandiridwa imakhala yoyera kwambiri ndipo ilibe zodetsa zamapuloteni.

Mphaka. Ayi	Kukulitsa Kukula
4992237	50 preps

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Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Zogwiritsira ntchito zabwino kwambiri pazitsanzo za mbeu zimapangitsa kuti ntchitoyi ikhale yosavuta.
■ DNase Wapadera I amachepetsa kuipitsidwa kwa DNA.
■ Mzere wapadera wa kusefera CS umachotsa zodetsa zina.
■ Kuyera kwa RNA kogwiritsa ntchito kwambiri ndi koyenera kugwiritsira ntchito mosavutikira.
■ Palibe chochotsa phenol / chloroform, palibe LiCl ndi mpweya wa ethanol, ndipo palibe CsCl gradients centrifugation yomwe ikufunika, zomwe zimapangitsa kuti ntchitoyi ikhale yotetezeka komanso yodalirika.

Mapulogalamu

■ RT-PCR.
■ Northern Blot, Dot Blot.
■ PCR Yeniyeni.
■ Kusanthula kwa Chip.
■ PolyA Screening, in vitro, cloning cloning.

Zindikirani

Ngati sampuli ili ndi metabolism yachiwiri yachiwiri, Buffer HL yoperekedwa ndi TIANGEN itha kugwiritsidwa ntchito kukwaniritsa kuyerekezera kwakukulu.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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Ena:

	Zakuthupi: 80 mg masamba a Atenia cordifolia Njira: RNA yonse yamasamba a Atenia cordifolia idadzipatula pogwiritsa ntchito RNAprep Pure Plant Kit. Zotsatira: Chonde onani chithunzi cha agarose gel electrophoresis pamwambapa. 2-4 μl of 100 μl eluates adakwezedwa pamsewu. Electrophoresis inkachitika ku
	Akuyerekeza zokolola za RNA za mitundu ingapo

Q: Kutsekeka kwamagawo

A-1 Cell lysis kapena homogenization sikokwanira

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa kuchuluka kwa nyemba zomwe mukugwiritsa ntchito kapena kuonjezera kuchuluka kwa lysis buffer.

Q: Zokolola zochepa za RNA

A-1 Kusakwanira kwa cell lysis kapena homogenization

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

- Chonde onani kuthekera kwakukulu kokonza.

A-3 RNA siyosankhidwa kwathunthu kuchokera pagawo

---- Mukatha kuwonjezera madzi opanda RNase, siyani kwa mphindi zochepa centrifuging.

A-4 Ethanol mumlengalenga

- Pambuyo kutsukidwa, centrifuge kachiwiri ndikuchotsa chotsuka chotsuka momwe zingathere.

A-5 Cell chikhalidwe sing'anga sichichotsedweratu

- Mukamasonkhanitsa maselo, chonde onetsetsani kuti muchotse sing'anga momwe mungathere.

A-6 Maselo osungidwa mu RNAstore sakhala otsekemera kwenikweni

---- RNAstore osalimba ndi wamkulu kuposa sing'anga wamba wamaselo; kotero mphamvu ya centrifugal iyenera kukulitsidwa. Akuti centrifuge pa 3000x g.

Zolemba za A-7 Low RNA komanso kuchuluka kwake mchitsanzo

- Gwiritsani ntchito chitsanzo chabwino kuti muwone ngati zokolola zochepa zimayambitsidwa ndi chitsanzocho.

Q: Kuwonongeka kwa RNA

A-1 Nkhaniyi siyatsopano

- Minyewa yatsopano imayenera kusungidwa mu nayitrogeni wamadzi nthawi yomweyo kapena kuyiyika mu reagent ya RNAstore kuti zitsimikizidwe kuti zimachokera.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-3 RNase kuipitsidwan

- Ngakhale gawo lotetezedwa mulibe RNase, ndikosavuta kuipitsa RNase panthawi yazochulukirapo ndipo iyenera kusamalidwa.

Kuwononga kwa A-4 Electrophoresis

---- Sinthanitsani cholumikizira cha electrophoresis ndikuwonetsetsa kuti zofunikira ndi Kutsegula Buffer zilibe vuto la RNase.

A-5 Kutsitsa kwambiri kwa electrophoresis

---- Kuchepetsa kuchuluka kwa potsegula, kutsitsa kwa chitsime chilichonse sikuyenera kupitirira 2 μg.

Q: kuipitsidwa kwa DNA

A-1 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-2 Zitsanzo zina zimakhala ndi DNA yambiri ndipo amatha kuchiritsidwa ndi DNase.

- Chitani chithandizo cha RNase-Free DNase ku yankho la RNA, ndipo RNA itha kugwiritsidwa ntchito mwachindunji poyeserera pambuyo pothandizidwa, kapena itha kuyeretsedwa ndi zida zoyeserera za RNA.

Q: Kodi mungachotse bwanji RNase pazomwe mungagwiritse ntchito poyesera ndi magalasi?

Zamagalasi, zophikidwa pa 150 ° C kwa 4 h. Pazitsulo zapulasitiki, zomizidwa mu 0,5 M NaOH kwa mphindi 10, kenako kutsukidwa bwino ndi madzi opanda RNase kenako ndikuwotcha kuti muchotse RNase. Ma reagents kapena mayankho omwe amagwiritsidwa ntchito poyeserera, makamaka madzi, ayenera kukhala opanda RNase. Gwiritsani ntchito madzi opanda RNase pamakonzedwe onse a reagent (onjezerani madzi mu botolo loyera lagalasi, onjezerani DEPC pamlingo womaliza wa 0,1% (V / V), gwedezani usiku umodzi ndi autoclave).

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