RNAprep Koyera Hi-Magazi Kit

Kuti muyeretsedwe ndi RNA yapamwamba komanso yokhazikika pamwazi.

RNAprep Pure Hi-Blood Kit imatulutsa bwino RNA yonse m'magazi atsopano ndi magazi omwe ali ndi ma anticoagulants angapo amitundu yosiyanasiyana. Zinthu za silicon matrix zomwe zimagwiritsidwa ntchito mgawo lomasulira ndi chinthu chatsopano chopangidwa ndi TIANGEN, chomwe chimafalitsa RNA moyenera, ndikuchotsa mapuloteni osayera kwambiri. RNA yotulutsidwa itha kugwiritsidwa ntchito m'mayeso osiyanasiyana akumunsi monga RT-PCR, RT-qPCR, kusanthula chip, kutsata kwapamwamba, Northern Blot, Dot Blot, PolyA kuwunika, kumasulira kwa vitro, kusanthula kwa RNase chitetezo, kupangika kwa ma molekyulu, ndi zina zambiri.

Mphaka. Ayi	Kukulitsa Kukula
4992903	50 preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Yoyenera magazi athunthu amitundu yosiyanasiyana, yosavuta kugwiritsa ntchito.
■ Kukhala ndi RNase-Free Filtration Column CS kuti muchotse bwino zosafunika.
■ Chikhazikitso chopangidwa mwapadera chitha kuonetsetsa kuti chotsitsa cha RNA ndichabwino komanso chokhazikika pamayeso osiyanasiyana akutsika.
■ Ntchito yotetezeka komanso yodalirika, sipafunika kutulutsa phenol / chloroform.

Mapulogalamu

RT-PCR, Northern Blot, RT-qPCR, kusanthula kwa chip, kuyendetsa bwino kwambiri, kuwunika kwa PolyA, kusanthula chitetezo cha RNase, kumasulira kwa vitro, ndi zina zambiri.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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Ena: RNA Easy Fast Tissue / Cell Kit

	Chithunzi 1. RNA yoyeretsedwa kuchokera ku 100 μl magazi amphaka atsopano mu ma anticoagulants osiyanasiyana ogwiritsa ntchito RNAprep Pure Hi-Blood Kit. 4-6 μl ya 50 μl eluates adakwezedwa pamsewu. M: TIANGEN DNA Chizindikiro III.
	Chithunzi 2. RNA yoyeretsedwa kuchokera ku 100 μl magazi atsopano a mbewa pogwiritsa ntchito RNAprep Pure Hi-Blood Kit. 4-6 μl ya 50 μl eluates adakwezedwa pamsewu. M: TIANGEN DNA Chizindikiro III.

Q: Kutsekeka kwamagawo

A-1 Cell lysis kapena homogenization sikokwanira

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa kuchuluka kwa nyemba zomwe mukugwiritsa ntchito kapena kuonjezera kuchuluka kwa lysis buffer.

Q: Zokolola zochepa za RNA

A-1 Kusakwanira kwa cell lysis kapena homogenization

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

- Chonde onani kuthekera kwakukulu kokonza.

A-3 RNA siyosankhidwa kwathunthu kuchokera pagawo

---- Mukatha kuwonjezera madzi opanda RNase, siyani kwa mphindi zochepa centrifuging.

A-4 Ethanol mumlengalenga

- Pambuyo kutsukidwa, centrifuge kachiwiri ndikuchotsa chotsuka chotsuka momwe zingathere.

A-5 Cell chikhalidwe sing'anga sichichotsedweratu

- Mukamasonkhanitsa maselo, chonde onetsetsani kuti muchotse sing'anga momwe mungathere.

A-6 Maselo osungidwa mu RNAstore sakhala otsekemera kwenikweni

---- RNAstore osalimba ndi wamkulu kuposa sing'anga wamba wamaselo; kotero mphamvu ya centrifugal iyenera kukulitsidwa. Akuti centrifuge pa 3000x g.

Zolemba za A-7 Low RNA komanso kuchuluka kwake mchitsanzo

- Gwiritsani ntchito chitsanzo chabwino kuti muwone ngati zokolola zochepa zimayambitsidwa ndi chitsanzocho.

Q: Kuwonongeka kwa RNA

A-1 Nkhaniyi siyatsopano

- Minyewa yatsopano imayenera kusungidwa mu nayitrogeni wamadzi nthawi yomweyo kapena kuyiyika mu reagent ya RNAstore kuti zitsimikizidwe kuti zimachokera.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-3 RNase kuipitsidwan

- Ngakhale gawo lotetezedwa mulibe RNase, ndikosavuta kuipitsa RNase panthawi yazochulukirapo ndipo iyenera kusamalidwa.

Kuwononga kwa A-4 Electrophoresis

---- Sinthanitsani cholumikizira cha electrophoresis ndikuwonetsetsa kuti zofunikira ndi Kutsegula Buffer zilibe vuto la RNase.

A-5 Kutsitsa kwambiri kwa electrophoresis

---- Kuchepetsa kuchuluka kwa potsegula, kutsitsa kwa chitsime chilichonse sikuyenera kupitirira 2 μg.

Q: kuipitsidwa kwa DNA

A-1 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-2 Zitsanzo zina zimakhala ndi DNA yambiri ndipo amatha kuchiritsidwa ndi DNase.

- Chitani chithandizo cha RNase-Free DNase ku yankho la RNA, ndipo RNA itha kugwiritsidwa ntchito mwachindunji poyeserera pambuyo pothandizidwa, kapena itha kuyeretsedwa ndi zida zoyeserera za RNA.

Q: Kodi mungachotse bwanji RNase pazomwe mungagwiritse ntchito poyesera ndi magalasi?

Zamagalasi, zophikidwa pa 150 ° C kwa 4 h. Pazitsulo zapulasitiki, zomizidwa mu 0,5 M NaOH kwa mphindi 10, kenako kutsukidwa bwino ndi madzi opanda RNase kenako ndikuwotcha kuti muchotse RNase. Ma reagents kapena mayankho omwe amagwiritsidwa ntchito poyeserera, makamaka madzi, ayenera kukhala opanda RNase. Gwiritsani ntchito madzi opanda RNase pamakonzedwe onse a reagent (onjezerani madzi mu botolo loyera lagalasi, onjezerani DEPC pamlingo womaliza wa 0,1% (V / V), gwedezani usiku umodzi ndi autoclave).

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