RNAprep Pure Cell/Bacteria Kit Featured Image

RNAprep Koyera Cell / Bacteria Kit

Kuyeretsa RNA yokwanira kuchokera kumaselo ndi mabakiteriya.

RNAprep Pure Cell / Bacteria Kit imapereka njira yofulumira, yosavuta komanso yotsika mtengo yoyeretsera RNA yathunthu kuchokera kumaselo otukuka ndi zitsanzo za mabakiteriya pogwiritsa ntchito gawo loyenda bwino komanso dongosolo lapadera lotetezera. Chikwamacho chimaphatikizapo RNase-Free Spin Column CR3 yoyeretsa RNA yapamwamba kwambiri pogwiritsa ntchito ukadaulo wa silika-membrane. Mtengo wapamwamba kwambiri wa RNA ukhoza kupezeka mumphindi 30-40 ndikudziyeretsa kwakukulu ndipo ulibe kuwonongeka kwa protein ndi genomic DNA.

Mphaka. Ayi	Kukulitsa Kukula
4992235	50 preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Ma buffer ndi ma protocol opangira maselo otukuka ndi mabakiteriya amapangitsa kuti ntchitoyi ikhale yosavuta komanso yosavuta.
■ DNase Wapadera I amachepetsa kuipitsidwa kwa DNA.
■ Ma Columns a RNase-Free Filtration Column amathetsa kuipitsidwa kwina.
■ RNA yoyera kwambiri komanso yogwiritsidwa ntchito ndiyabwino kuchitira tcheru kumapeto.
■ Palibe chochotsa phenol / chloroform, palibe LiCl ndi mpweya wa ethanol, palibe CsCl gradient centrifugation yomwe ikufunika, zomwe zimapangitsa kuti ntchitoyi ikhale yotetezeka komanso yodalirika.

Mapulogalamu

■ RT-PCR.
■ Northern Blot, Dot Blot.
■ PCR Yeniyeni.
■ Kusanthula kwa Chip.
■ Kuwunika kwa PolyA, kumasulira kwa ma vitro, kusanthula kwa RNase ndi kupangika kwa ma molekyulu.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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Ena: Chida Choyera cha RNAprep Kit

	Zakuthupi: Maselo a Jurkat Amunthu (1 × 10⁶ ) Njira: RNA yathunthu ya Maselo a Jukat aanthu adadzipatula pogwiritsa ntchito RNAprep Pure Cell / Bacteria Kit. Zotsatira: Chonde onani chithunzi cha agarose gel electrophoresis pamwambapa. 2-4 μl a 50 μl eluates adakwezedwa pamsewu. Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose gel.
	Zakuthupi: TOP10 E.coli (1 × 10⁸) Njira: RNA yonse ya TOP10 E.coli idadzipatula pogwiritsa ntchito RNAprep Pure Cell / Bacteria Kit. Zotsatira: Chonde onani chithunzi cha agarose gel electrophoresis pamwambapa. 2-4 μl a 50 μl eluates adakwezedwa pamsewu. Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose gel.

Q: Kutsekeka kwamagawo

A-1 Cell lysis kapena homogenization sikokwanira

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa kuchuluka kwa nyemba zomwe mukugwiritsa ntchito kapena kuonjezera kuchuluka kwa lysis buffer.

Q: Zokolola zochepa za RNA

A-1 Kusakwanira kwa cell lysis kapena homogenization

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

- Chonde onani kuthekera kwakukulu kokonza.

A-3 RNA siyosankhidwa kwathunthu kuchokera pagawo

---- Mukatha kuwonjezera madzi opanda RNase, siyani kwa mphindi zochepa centrifuging.

A-4 Ethanol mumlengalenga

- Pambuyo kutsukidwa, centrifuge kachiwiri ndikuchotsa chotsuka chotsuka momwe zingathere.

A-5 Cell chikhalidwe sing'anga sichichotsedweratu

- Mukamasonkhanitsa maselo, chonde onetsetsani kuti muchotse sing'anga momwe mungathere.

A-6 Maselo osungidwa mu RNAstore sakhala otsekemera kwenikweni

---- RNAstore osalimba ndi wamkulu kuposa sing'anga wamba wamaselo; kotero mphamvu ya centrifugal iyenera kukulitsidwa. Akuti centrifuge pa 3000x g.

Zolemba za A-7 Low RNA komanso kuchuluka kwake mchitsanzo

- Gwiritsani ntchito chitsanzo chabwino kuti muwone ngati zokolola zochepa zimayambitsidwa ndi chitsanzocho.

Q: Kuwonongeka kwa RNA

A-1 Nkhaniyi siyatsopano

- Minyewa yatsopano imayenera kusungidwa mu nayitrogeni wamadzi nthawi yomweyo kapena kuyiyika mu reagent ya RNAstore kuti zitsimikizidwe kuti zimachokera.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-3 RNase kuipitsidwan

- Ngakhale gawo lotetezedwa mulibe RNase, ndikosavuta kuipitsa RNase panthawi yazochulukirapo ndipo iyenera kusamalidwa.

Kuwononga kwa A-4 Electrophoresis

---- Sinthanitsani cholumikizira cha electrophoresis ndikuwonetsetsa kuti zofunikira ndi Kutsegula Buffer zilibe vuto la RNase.

A-5 Kutsitsa kwambiri kwa electrophoresis

---- Kuchepetsa kuchuluka kwa potsegula, kutsitsa kwa chitsime chilichonse sikuyenera kupitirira 2 μg.

Q: kuipitsidwa kwa DNA

A-1 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-2 Zitsanzo zina zimakhala ndi DNA yambiri ndipo amatha kuchiritsidwa ndi DNase.

- Chitani chithandizo cha RNase-Free DNase ku yankho la RNA, ndipo RNA itha kugwiritsidwa ntchito mwachindunji poyeserera pambuyo pothandizidwa, kapena itha kuyeretsedwa ndi zida zoyeserera za RNA.

Q: Kodi mungachotse bwanji RNase pazomwe mungagwiritse ntchito poyesera ndi magalasi?

Zamagalasi, zophikidwa pa 150 ° C kwa 4 h. Pazitsulo zapulasitiki, zomizidwa mu 0,5 M NaOH kwa mphindi 10, kenako kutsukidwa bwino ndi madzi opanda RNase kenako ndikuwotcha kuti muchotse RNase. Ma reagents kapena mayankho omwe amagwiritsidwa ntchito poyeserera, makamaka madzi, ayenera kukhala opanda RNase. Gwiritsani ntchito madzi opanda RNase pamakonzedwe onse a reagent (onjezerani madzi mu botolo loyera lagalasi, onjezerani DEPC pamlingo womaliza wa 0,1% (V / V), gwedezani usiku umodzi ndi autoclave).

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