■ Chitetezo chokwanira: Tetezani RNA m'magazi atsopano kuti asawonongeke.
■ Yabwino: Opaleshoniyo imangotenga masitepe awiri okha ndipo ndiyabwino kusunga magawo ambiri amwazi.
■ Kugwirizana kwathunthu: Zimagwirizana ndi zida za TIANGEN za kampani ya Silicon Matrix Membrane Yotsuka kuti itulutse magazi a RNA kapena DNA kuti zitsimikizire zokolola ndi kuyera.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
Zakuthupi: 100 μl magazi atsopano a mbewa Njira: RNA yathunthu yamagazi atsopano a mbewa (osungidwa mu RNALock Reagent) omwe amasungidwa pa 15-25 ℃, 4-8 ℃, -20 / -70 ℃ adadzipatula pogwiritsa ntchito RNAprep Pure Blood Kit (4992238) kutsatira RNALock protocol . Zotsatira: Chonde onani chithunzi cha agarose gel electrophoresis pamwambapa. 4-6 μl ya 50 μl eluates adakwezedwa pamsewu. M: TIANGEN DNA Chizindikiro III; Njira 1-2 (kuwongolera koyenera): RNA yoyeretsedwa kuchokera kumagazi atsopano; Lane 3-4: RNA yoyeretsedwa kuchokera kumagazi omwe amasungidwa 15-25 ℃ masiku awiri. Njira 5-6: RNA yoyeretsedwa kuchokera kumagazi omwe amasungidwa 4-8 ℃ sabata limodzi. Njira 7-8: RNA yoyeretsedwa kuchokera kumagazi omwe amasungidwa pa -20 ℃ kapena -70 ℃ kwa theka la chaka. Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose. |
Chiyambireni kukhazikitsidwa, fakitole yathu yakhala ikupanga zinthu zoyambirira motsatira mfundoyo
yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..