Ultra-pure HotStart yodalirika kwambiri yotheka ndi DNA polymerase.
Taq DNA polymerase yoyera komanso yoyera.
Zochita zapamwamba kwambiri komanso mwatsatanetsatane Taq DNA polymerase.
Njira zosavuta komanso zowonekera bwino za RT-PCR reagent.
Kuchotsa kwa gDNA ndikusinthira mawu mumphindi 18.
Werengani bwino mitundu yonse yazotsatizana ndikuzindikira molondola ma tempuleti ochepa.
Yoyenera ma tempuleti okhala ndi zomangamanga zovuta komanso kaphatikizidwe kabwino ka cDNA yayitali.
Kuthamanga kwachangu komanso kogwira ntchito mosasunthika pa 50 ng-2 μg ya RNA yathunthu.
Kukhulupirika kwambiri, kutsimikizika kwapamwamba komanso kuyendetsa bwino PCR koyambirira.
Kukhulupirika kopanda tanthauzo la Taq DNA polymerase.
Kukulitsa mwachangu mtundu wa chandamale mwachindunji kuchokera kuzitsanzo zamatenda a nyama popanda kutulutsa.
Chiyambireni kukhazikitsidwa, fakitole yathu yakhala ikupanga zinthu zoyambirira motsatira mfundoyo yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..