Njira ya mNGS

Tekinoloje yodziwitsa za maselo, makamaka tizilombo toyambitsa matenda metagenomic detection (mNGS), ili ndi chiyembekezo chabwino chogwiritsa ntchito matenda opatsirana pogonana, chizindikiritso chatsopano chosadziwika, matenda opatsirana, mankhwala osokoneza bongo, kuwunika momwe anthu amagwirira ntchito ndikuwunika mphamvu yothandizira matenda. Imakhala ndi njira yofunikira popewa komanso kuwongolera matenda opatsirana, komanso imalimbikitsa kupititsa patsogolo luso lazachipatala.
MNGS imatha kupenda mozama ma microbial and host genetic material (DNA ndi RNA) mu zitsanzo za odwala, ndipo pang'onopang'ono amasamutsidwa kuchoka ku labotale kupita kuchipatala, ngati gawo limodzi lodziwitsa kusiyanasiyana kwa madotolo, ndikuthandizira kuthandizira kuzindikira.
TIANGEN imapereka yankho pakuchotsa koyambirira kwa tizilombo ting'onoting'ono komanso zomangamanga za mNGS.

Kuyankha ku mliri wa COVID-19

Kuyambira pomwe COVID-19 idatuluka, TIANGEN yapereka mayeso a 5 mamiliyoni ngati zida zopangira ma virus a acid nucleic acid ndi ma fluorentient reagents opezera opanga opitilira 200 opanga ma reagent ndi mayunitsi ozindikira ku Asia, North ndi South America, ndi mayiko aku Europe. Ndipo ikuthandiza anthu mamiliyoni ambiri m'maiko opitilira 30 padziko lonse lapansi.

Zida zaku TIANGEN zochotsa ma virus, monga zopangira, zidadziwika mu lipoti lowunikira zakugwiritsa ntchito mwadzidzidzi kwa COVID-19 yotulutsidwa ndi World Health Organisation mu Juni 2020, adatchulidwa pamndandanda wovomerezeka wa zida zatsopano zodziwika za COVID-19 zotulutsidwa ndi Global Fund mu Januwale 2021.

Mankhwala Quality Control

Kuyambira zopangira kumaliza mankhwala
Maulalo onse amayang'aniridwa moyenera ndi ISO13485 kasamalidwe kabwino

Production Environment

Malo Opanga

Raw Materials

Zida zogwiritsira ntchito

Semi-products

Zotuluka

QC-NGS-Based

Chotsatira cha QC-NGS

Njira Yonse ya mNGS

Zitsanzo Zosunga

Zitsanzo Pretreatment

Kuchotsa Kwama acid

NGS

Zitsanzo Zosunga

Oral swab yoteteza Preservation Buffer

ZOKHUDZA KWAMBIRI

Tetezani RNA osazizira
Ntchito: ubongo, mtima, impso, ndulu, chiwindi, mapapo ndi thymus, ndi zina zambiri.

Amasunga RNA: 1 tsiku 37 ° C, masiku 7 pa 15-25 ° C, kapena masabata 4 pa 2-8 ° C. -20 ° C kapena -80 ° C

Zitsanzo Pretreatment

Matenda a nyemba akupera

Njira Yothetsera Anthu

Ndioyenera kutulutsa DNA / RNA / mapuloteni ochokera kumatumba / zinyama, nthaka, ndowe, bowa, ndi zina zambiri.

Kutentha kotentha: mpaka -10 ℃
Kutulutsa: zitsanzo 1-24

Zotsatira za RNA yanyama yomwe imachotsedwa pambuyo pogaya ndi H24R

sss

Zitsanzo zazing'ono: 20 mg (mtima: 10mg) wa mkulu ndende ndi chiyero.

Kuchotsa Kwama acid

Yodzichitira Nucleic Acid Sola Series

● Njira 32- ndi 96 zosankha.

● Kutulutsa mwachangu kachilombo ka acid mkati mwa mphindi 30.

● Makina opanga ma reagent apamwamba omwe amapezeka kuti agwire bwino ntchito.

Open High matulukidwe Anakonza

Maginito Hi-DNA / RNA Kit (4992408 -T4A)

● Kugwirizana kwakukulu, kofananira bwino komwe kumapezeka pamsika.

● Zogulitsa mwadongosolo komanso ntchito za OEM zimapangidwa molingana ndi zosowa zina.

● Zimayenderana: KingFisher, Hamilton, Beckman Coulter, Chemagen etc.

Real time PCR amplifi cation curve of TIANGEN DP438

Nthawi yeniyeni ya PCR amplifi cation curve ya TIANGEN 4992408

Real time PCR amplifi cation curve of Supplier T

Nthawi yeniyeni ya PCR amplifi cation curve ya Supplier T

AIV-H5 idasinthidwa kukhala 10-6 -10-8ma gradients okhala ndi Milli-Q madzi, kenako otengedwa ndi King Fisher Flex. 200 μl pachitsanzo chilichonse adagwiritsidwa ntchito. Zida za TIANGEN zikuwonetsa chidwi komanso kukhazikika. Chida cha PCR chenicheni: ABI 7500 Real-time PCR kudziwika reagent: FP314

Anakonza m'zigawo Buku

● Pakufunika zida zosavuta kugwiritsa ntchito pomaliza kuyesa

● Zogwiritsa ntchito zimadzaza mosiyana kuti zisawonongeke

● Kufupikitsa nthawi ndi ntchito yosavuta, yogwira bwino ntchito.

● Zogulitsa mwadongosolo komanso ntchito za OEM zimapangidwa molingana ndi zosowa zina.

TIANamp Virus DNA / RNA Kit (4992285)

TIANamp Virus RNA Kit (4992286)

TIANamp Micro DNA Kit (4992287)

Mphaka. Mtundu wa asidi wa nyukiliya Mtundu wogwiritsira ntchito
4992285 DNA / RNA Seramu, plasma, madzimadzi amthupi, minofu, swab kuteteza njira, ma virus pachikhalidwe, ndi zina zambiri
4992286 RNA Seramu, plasma, madzimadzi amthupi, minofu, swab kuteteza njira, ma virus pachikhalidwe, ndi zina zambiri
4992287 DNA Mabakiteriya, bowa, majeremusi ndi ma virus omwe amakhala kutali ndi seramu, plasma, minofu, pleural ndi ascites, cerebrospinal fluid, sputum, bronchoalveolar lavage fluid ndi paraffin

mNGS Kuzindikira Kukonzekera Laibulale

title (3)

● TIANSeq DirectFast Library Kit (illumina) (4992259/4992260)
Kugawanika kwa Enzymatic DNA. Yoyenera kumangidwe kwa laibulale ya DNA ya nsanja yolumikizira kwambiri ya Illumina

● TIANSeq Fast DNA Library Kit (illumina) (4992261/4992262)
Yoyenera kumangidwe kwa laibulale ya DNA ya nsanja yolumikizira kwambiri ya Illumina

● Fast DNA Library Kit ya Ion Torrent Platform (Yokonda zida)
Yoyenera kumangidwe kwa laibulale ya DNA ya Ion torrent high throughput sequential platform

● Fast DNA Library Kit ya MGI Platform (Makonda zida)
Oyenera DNA yomanga laibulale MGI mkulu matulukidwe nsanja nsanja

title (1)

● TIANSeq rRNA Depletion Kit (H / M / R) (4992363/4992364/4992391) (ya nsanja za Illumina / Ion / MGI)
Kuchotsa rRNA wolandirayo kuti athandize kuzindikira kwa ma virus a RNA

title (2)

● TIANSeq Fast RNA Library Prep Kit (Illumina) (4992375)
Pofuna kupanga laibulale ya virus ya RNA mutachotsa rRNA yomwe ikuthandizira, yomwe ingadziwe mwachangu kupezeka kwa ma virus a RNA

● TIANSeq Anasunthika RNA-Seq Kit (Illumina) (4993007)
Pofuna kupanga laibulale ya RNA yamafuta atachotsa rRNA, yomwe imatha kudziwa molondola kusiyana kwa ma virus

● RNA RNA Library yokonzekera Kit ya MGI Platform (Yokonzedwa mwamakonda)
Yoyenera kumangidwe kwa laibulale ya RNA pa pulatifomu yoyendetsa bwino ya MGI

title (1)

● TIANSeq Kukula Kusankhidwa kwa DNA mikanda (4992358/4992359/4992979)
Poyeretsa ndikusankha kukula kwa zidutswa za DNA panthawi yopanga laibulale ya DNA

● TIANSeq RNA Woyera Makanda (4992360/4992362/4992867)
Kuyeretsa kwa RNA pambuyo pakupindulitsa kwa RNA

Mayankho onse pamwamba akhoza makonda kwa ODM / OEM. Kuti mudziwe zambiri, chonde dinani