Maginito Nthaka Ndi chopondapo DNA zida
Mawonekedwe
■ Kugwiritsa ntchito kwakukulu: Yoyenera kutulutsa mitundu ingapo yamiyeso yazachilengedwe monga nthaka ya maluwa, dothi la mphika, nthaka ya minda, nkhalango zamapiri, silt, nthaka yofiira, nthaka yakuda, fumbi ndi zina zotero. Iyenso ndi yoyenera kutulutsa zitsanzo zampando ndi tizilombo toyambitsa matenda.
■ Ntchito yabwino: Ntchito yoyesayi imatha kumaliza nthawi yochepa.
■ Kuyera kwambiri: Kuphatikiza ndi kuyeretsedwa kwa maginito, DNA yotulutsidwa imakhala yoyera kwambiri ndipo itha kugwiritsidwa ntchito mwachindunji poyesa kutsika
Mapulogalamu
DNA yoyeretsedwa ndi chidacho ili ndi zodetsa zochepa komanso umphumphu wabwino, ndipo itha kugwiritsidwa ntchito mwachindunji m'mayeso ena otsika a biology ya maselo monga PCR, chimbudzi chimbudzi, ndi zina zambiri.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
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Genomic DNA idatengedwa kuchokera ku 500 mg wamunda wam'munda ndi wogwedeza komanso wopukusira motsatana, pogwiritsa ntchito TIANGEN Magnetic Soil And Stool DNA Kit ndi chinthu choyenera kuchokera ku Supplier M. 5 μl ya 100 μl eluate chidakwezedwa 1% agarose gel electrophoresis. M: Wopanga TIANGEN D2000 |
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Genomic DNA idachotsedwa m'mipando ya anthu ndi TIANGEN Magnetic Soil And Stool DNA Kit ndi chinthu choyenera kuchokera kwa Supplier M motsatana, ndipo idadziwika ndi PCR yokhala ndi ma 16S oyambitsa mabakiteriya. 5 μl ya 20 μl PCR mankhwala adayikidwa 1% agarose gel electrophoresis. M: Wopanga TIANGEN D2000 |
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Genomic DNA idapangidwa kuchokera kuzilombo zazing'ono zam'madzi za nsomba pogwiritsa ntchito TIANGEN Magnetic Soil And Stool DNA Kit ndi chinthu chofunikira kuchokera ku Supplier M motsatana, ndipo chidapezeka ndi PCR chokhala ndi bakiteriya wamkulu wa 27F / 1492R wokhala ndi mankhwala pafupifupi 1500 bp. 5 μl ya 20 μl PCR mankhwala adayikidwa 1% agarose gel electrophoresis. M: Wopanga TIANGEN D2000 |
A-1 Kuchuluka kwa maselo kapena kachilombo koyambitsa nyereredwe yoyamba - Limbikitsani kuchuluka kwa ma cell kapena ma virus.
A-2 Kuperewera kwa sampuli -Zitsanzozo sizinasakanikirane bwino ndi chosungira cha lysis. Amalangizidwa kuti azisakanikirana bwino ndi kutentha kwa thupi maulendo 1-2. —Maselo osakwanira omwe amabwera chifukwa cha kuchepa kwa proteinase K. —Wosakwanira cell lysis kapena kuwonongeka kwa mapuloteni chifukwa chosakwanira nthawi yosamba. Amalangizidwa kuti azidula zidutswazo tizidutswa tating'ono ndikuwonjezera nthawi yosamba kuti achotse zotsala zonse mu lysate.
A-3 Kusakwanira kwa DNA kutsatsa. - Palibe ethanol kapena peresenti yotsika m'malo mwa 100% ethanol yomwe idawonjezeredwa lysate isanatumizidwe ku gawo loyambira.
A-4 Mtengo wa pH wa elution buffer ndiwotsika kwambiri. -Sinthani pH kuti ikhale pakati pa 8.0-8.3.
Mowa wotsalira mumtambo.
—Pali zotsalira zotsukira PW mumlengalenga. Mowa ungachotsedwe poyika gawo loyenda la 3-5 min, kenako ndikuyika kutentha kapena 50 ℃ incubator ya 1-2 min.
A-1 Chitsanzocho sichatsopano. —Tengani chitsanzo chabwino cha DNA kuti muwone ngati DNA yomwe ili mchitsanzocho yawonongeka.
A-2 Chithandizo chisanachitike. —Kumachitika chifukwa cha utsi wambiri wa nayitrogeni, kupezanso chinyezi, kapena kuchuluka kwa chitsanzocho.
Zoyesererazi ziyenera kusiyanasiyana pamitundu yosiyanasiyana. Pazitsamba, onetsetsani kuti mukugaya madzi asafe. Zitsanzo za nyama, homogenate kapena sungani bwino madzi asafe. Pazitsanzo zokhala ndi makoma osavuta kuswa, monga mabakiteriya a G + ndi yisiti, amalimbikitsidwa kugwiritsa ntchito lysozyme, lyticase kapena njira zamakina kuti athyole makoma am'maselo.
4992201/4992202 Plant Genomic DNA Kit imagwiritsa ntchito njira yozikika yomwe imafunikira chloroform kuti izichotse. Makamaka ndi oyenera mitundu yazomera zosiyanasiyana, komanso ufa wouma wouma. Hi-DNAsecure Plant Kit imapangidwanso m'mbali, koma osafunikira kuchotsedwa kwa phenol / chloroform, kuti ikhale yotetezeka komanso yopanda poizoni. Ndioyenera kumera ndi polysaccharides komanso polyphenol. 4992709/4992710 DNAquick Plant System imagwiritsa ntchito njira yopangira madzi. Kuchotsa kwa phenol / chloroform sikufunikanso. Njira yodziyeretsera ndiyosavuta komanso yachangu yopanda malire pazitsanzo zoyambira, kotero ogwiritsa ntchito amatha kusintha ndalamazo mosinthasintha malinga ndi zoyeserera. Kukula kwakukulu kwa zidutswa za gDNA kumatha kupezeka ndi zokolola zambiri.
DNA ya genomic idatengedwa m'magulu osiyanasiyana amwazi ndi TIANamp Blood DNA Kit. Zotsatira zake ndi izi. Zotsatirazo zalembedwa ngati zolembedwera zokha, zotsatira zake zenizeni zimadalira momwe zitsanzo zilili.
Kutulutsa magazi kwamagazi a DNA kumatha kuchitidwa pogwiritsa ntchito ma reagents omwe amapezeka mu zida ziwirizi posintha ndondomekoyi pamalangizo apadera otulutsa magazi a DNA. Kope lofewa la pulogalamu yamagazi yamagazi ya DNA limatha kuperekedwa mukapempha.
Yimitsani zitsanzo zatsopano ndi 1 ml PBS, saline wamba kapena buff buff. Kwathunthu homogenize nyemba ndi homogenizer ndi kusonkhanitsa precipitate pansi pa chubu ndi centrifuging. Tayani supernatant, ndikuyambiranso kuyambiranso ndi 200 μl buffer GA. Kuyeretsa kwa DNA kungachitike potsatira malangizo.
Poyeretsa gDNA mu plasma, seramu ndi madzi madzimadzi, TIANamp Micro DNA Kit ikulimbikitsidwa. Poyeretsa kachilombo ka HIV kuchokera ku zitsanzo za seramu / plasma, TIANamp Virus DNA / RNA Kit ikulimbikitsidwa. Poyeretsa bakiteriya gDNA kuchokera ku ma seramu ndi zitsanzo za plasma, TIANamp Bacteria DNA Kit ikulimbikitsidwa (lysozyme iyenera kuphatikizidwa ndi bakiteriya wabwino). Mwa zitsanzo za malovu, Hi-Swab DNA Kit ndi TIANamp Bacteria DNA Kit amalimbikitsidwa.
Chida chodzikongoletsera cha DNAsec kapena DNAquick Plant System ndikulimbikitsidwa pakuchotsa mafangasi a genome. Pochotsa yisiti, TIANamp Yisiti ya Kit ikulimbikitsidwa (lyticase iyenera kudzikonzekeretsa).
Zamgululi siyana
N'CHIFUKWA SANKHANI US
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yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..
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