DNA Bisulfite Conversion Kit Featured Image

Kutembenuka kwa DNA Bisulfite

Kutembenuka ndi kuyeretsedwa kumatha kumaliza mu ola la 2, ndikusintha mpaka 99%.

DNA Bisulfite Conversion Kit idapangidwa mwapadera kuti DNA isinthe bisulfite mu kafukufuku wa DNA methylation. Kuchiza kwa zitsanzo za DNA pogwiritsa ntchito chida ichi kumatha kumaliza maola awiri, ndipo kuchuluka kwa cytosine yosasunthika kukhala uracil kwatha 99%. Chida ichi chimatenga gawo lapadera la zoteteza DNA, lomwe limatsimikizira kuti DNA ndiyabwino komanso itachira ikatembenuka. Kuphatikiza apo, chida ichi chimagwiritsanso ntchito njira yochotsera gulu la asidi sulphurous acid, zomwe zimapangitsa kuti ntchito yonse ikhale yosavuta.

Mphaka. Ayi	Kukulitsa Kukula
4992447	50 preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Zosavuta komanso zachangu: Kusintha ndi kuyeretsa kumatha kumaliza maola awiri, ndizofunikira zochepa pazida ndi zida, zomwe ndizoyenera mabungwe ofufuza m'magulu onse.
■ Kutembenuka kwakukulu: Kusintha kwa cytosine yosasunthika kukhala uracil mu sampuli ya DNA kwadutsa 99%.
■ Kuzindikira kwambiri: Chidachi chimatha kusanja ma sampuli a DNA osachepera 500 pg mpaka 2.5 μg.

Mfundo

Mtundu: Kutembenuka kwa Bisulfite
Zitsanzo za DNA: 500 pg-2.5 μg
Mtengo wosinthitsira > 99%
Nthawi yogwiritsira ntchito: ~ 2 ora
Mapulogalamu: Zitsanzo za DNA zomwe zimagwiritsidwa ntchito ndi chida ichi ndizoyenera PCR / qPCR ya methylation, kusanja ndi ma microarray.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

Previous: Zamgululi TIANSeq mRNA Capture Kit

Ena: Chida cha Methylation-specipc PCR (MSP)

Gulu 1: Kutembenuka kwa "C" kosasinthidwa kukhala "U"
Pogwiritsa ntchito matupi athu monga zinthu zoyesera, kuchuluka kwa chitsanzocho kumatha kufikira 65%, ndipo kuchuluka kwa "C" kosagwiritsidwa ntchito ngati "U" kumatha kufikira 99%.

Table 1: Conversion rate of unmethylated “C” to “U” Using human genome as experimental material, the recovery rate of the sample can reach 65%, and the ratio of unmethylated “C” to “U” can reach more than 99%.