TRNzol Universal Reagent

Njira yatsopano yosinthira mitundu ingapo yosintha.

TRNzol Universal Reagent itha kugwiritsidwa ntchito kupatula RNA yathunthu kuchokera kuzitsanzo monga mavairasi, mabakiteriya, bowa, nyama, minofu yazinyama ndi madzi amthupi okhala ndi kuthekera kwa lysis komanso kuzindikira kwambiri. Imasunga umphumphu wa RNA pomwe imasokoneza maselo ndikusungunula magawo am'maselo panthawi yopanga homogenization. RNA yokhayokha ndi chida ichi imatha kugwira ntchito ngati ma tempuleti azidziwitso zakumunsi kapena ntchito zina.

Mphaka. Ayi	Kukulitsa Kukula
4992730	100 ml ya

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Kusinthasintha kwakukulu: Kutalika koyambira koyambira koyambira, koyenera kutulutsa sampuli yayikulu poyankha kamodzi.
■ Zokolola zambiri: Njira yampweya imakulitsa zokolola za RNA muzitsanzo.
Kugwiritsa ntchito kwambiri: Oyenera mitundu yambiri yosiyanasiyana monga minofu yazinyama ndi nyama, maselo otukuka, magazi, madzi amthupi, ndi zina zambiri.
■ Kugwira ntchito mwachangu: Genomic DNA itha kupezeka mkati mwa ola limodzi.

Mfundo

Mtundu: Mvula yochokera
Zitsanzo: Mavairasi, mabakiteriya, bowa, nyama, minofu yazomera, maselo otukuka ndi madzi amthupi.
Chandamale: RNA
Nthawi yogwiritsira ntchito: ~ Ola limodzi
Mapulogalamu: TRNzol Universal reagent imachepetsa kuipitsidwa kwa zosayera monga DNA ndi mapuloteni mu RNA yoyeretsedwa, ndipo itha kugwiritsidwa ntchito mwachindunji pakuyesa kwamitundu yosiyanasiyana ya biology monga Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, cDNA library yomanga , RT-PCR, nthawi yeniyeni ya PCR komanso mayendedwe apamwamba.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

Previous: Zamgululi RNAprep Koyera yaying'ono zida

Ena: RNAprep Koyera Cell / Bacteria Kit

Workflow

Njira: 30 mg makoswe a chiwindi, 100 mg masamba a mpunga adasonkhanitsidwa ndikupera madzi asafe; 1 × 10⁶Maselo otukuka a HepG2 ndi 700 μl Saccharomyces Cerevisiae medium medium (OD600 = 0.9) adasonkhanitsidwa ndi centrifugation. 1 ml ya TRNzol Universal Reagent yochokera ku TIANGEN ndi zinthu zofunikira kuchokera kwa omwe amapereka L ndi T zidawonjezedwa ku aliquot iliyonse yazitsanzo ndipo kutulutsa kwa RNA kunkachitika potsatira ndondomeko zoperekedwa ndi wopereka aliyense. Voliyumu ya elution inali 80 μl, 50 μl, 30 μl ndi 30 μl pazitsanzo zinayi motsatira. 3 μl ya eluate idayikidwa pamseu uliwonse.
MIII: TIANGEN Marker III;
Electrophoresis inkachitika pa 6 V / cm kwa mphindi 30 pa 1% agarose.
Zotsatira: TIANGEN TRNzol Universal Reagent imatha kuchotsa kuyera kwambiri ndi umphumphu wabwino RNA kuchokera ku chiwindi cha khoswe, masamba a mpunga, maselo otukuka ndi zitsanzo za yisiti, moyenera kwambiri. Mtengo wa RNA ndi wofanana kapena wokwera pang'ono kuposa womwe umapereka kwa L ndi T.

Q: Kutsekeka kwamagawo

A-1 Cell lysis kapena homogenization sikokwanira

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa kuchuluka kwa nyemba zomwe mukugwiritsa ntchito kapena kuonjezera kuchuluka kwa lysis buffer.

Q: Zokolola zochepa za RNA

A-1 Kusakwanira kwa cell lysis kapena homogenization

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

- Chonde onani kuthekera kwakukulu kokonza.

A-3 RNA siyosankhidwa kwathunthu kuchokera pagawo

---- Mukatha kuwonjezera madzi opanda RNase, siyani kwa mphindi zochepa centrifuging.

A-4 Ethanol mumlengalenga

- Pambuyo kutsukidwa, centrifuge kachiwiri ndikuchotsa chotsuka chotsuka momwe zingathere.

A-5 Cell chikhalidwe sing'anga sichichotsedweratu

- Mukamasonkhanitsa maselo, chonde onetsetsani kuti muchotse sing'anga momwe mungathere.

A-6 Maselo osungidwa mu RNAstore sakhala otsekemera kwenikweni

---- RNAstore osalimba ndi wamkulu kuposa sing'anga wamba wamaselo; kotero mphamvu ya centrifugal iyenera kukulitsidwa. Akuti centrifuge pa 3000x g.

Zolemba za A-7 Low RNA komanso kuchuluka kwake mchitsanzo

- Gwiritsani ntchito chitsanzo chabwino kuti muwone ngati zokolola zochepa zimayambitsidwa ndi chitsanzocho.

Q: Kuwonongeka kwa RNA

A-1 Nkhaniyi siyatsopano

- Minyewa yatsopano imayenera kusungidwa mu nayitrogeni wamadzi nthawi yomweyo kapena kuyiyika mu reagent ya RNAstore kuti zitsimikizidwe kuti zimachokera.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-3 RNase kuipitsidwan

- Ngakhale gawo lotetezedwa mulibe RNase, ndikosavuta kuipitsa RNase panthawi yazochulukirapo ndipo iyenera kusamalidwa.

Kuwononga kwa A-4 Electrophoresis

---- Sinthanitsani cholumikizira cha electrophoresis ndikuwonetsetsa kuti zofunikira ndi Kutsegula Buffer zilibe vuto la RNase.

A-5 Kutsitsa kwambiri kwa electrophoresis

---- Kuchepetsa kuchuluka kwa potsegula, kutsitsa kwa chitsime chilichonse sikuyenera kupitirira 2 μg.

Q: kuipitsidwa kwa DNA

A-1 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-2 Zitsanzo zina zimakhala ndi DNA yambiri ndipo amatha kuchiritsidwa ndi DNase.

- Chitani chithandizo cha RNase-Free DNase ku yankho la RNA, ndipo RNA itha kugwiritsidwa ntchito mwachindunji poyeserera pambuyo pothandizidwa, kapena itha kuyeretsedwa ndi zida zoyeserera za RNA.

Q: Kodi mungachotse bwanji RNase pazomwe mungagwiritse ntchito poyesera ndi magalasi?

Zamagalasi, zophikidwa pa 150 ° C kwa 4 h. Pazitsulo zapulasitiki, zomizidwa mu 0,5 M NaOH kwa mphindi 10, kenako kutsukidwa bwino ndi madzi opanda RNase kenako ndikuwotcha kuti muchotse RNase. Ma reagents kapena mayankho omwe amagwiritsidwa ntchito poyeserera, makamaka madzi, ayenera kukhala opanda RNase. Gwiritsani ntchito madzi opanda RNase pamakonzedwe onse a reagent (onjezerani madzi mu botolo loyera lagalasi, onjezerani DEPC pamlingo womaliza wa 0,1% (V / V), gwedezani usiku umodzi ndi autoclave).

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