■ Yosavuta kugwiritsa ntchito: Ntchito yamapulogalamuyi imatha kuzindikirika mosavuta mkati mwa 1 h. Dongosolo lokonzekera la PCR / qPCR lokhoza kusinthidwa ndikusinthidwa mwachangu.
■ Zogwiritsira ntchito zogwirizana: Chida chosinthira cha pipette ndi dongosolo la Beckman Biomek 3000.
■ Kukonza kosavuta: Module yapaipetting module (APM) itha kusinthidwa mosavuta ndikutumizidwanso kuti ikonze zolakwika.
■ Kulondola kwambiri komanso kulondola.
Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)
Chithunzi 1: Zotsatira zokhazikika za qPCR zikuwonetsa kubwereza kwabwino Zitsanzo za cDNA za 7 μl NIH 3T3 zimasungunuka nthawi 4 ndi 21 μl amadzi pamiyeso ya 1: 4. Roche LightCycler 480 real-time fluorescence kuchuluka kwa PCR chida ndi SuperReal PreMix Plus (SYBR Green) adagwiritsidwa ntchito pozindikira. |
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Chithunzi 2: Kulondola kwambiri poyerekeza ndi kupopera kwa manja (Kumanzere: Buku; Kumanja: KUSINTHA KWA MPHAMVU) 4 zobwereza za GAPDH ya anthu yamphamvu (pamwamba pamapindikira). Onjezani 2 μl cDNA mpaka 18 μl MasterMix kuti mupange 20 μl reaction system. Roche LightCycler 480 real-time fluorescence kuchuluka kwa PCR chida ndi SuperReal PreMix Plus (SYBR Green) adagwiritsidwa ntchito pozindikira. |
Chiyambireni kukhazikitsidwa, fakitole yathu yakhala ikupanga zinthu zoyambirira motsatira mfundoyo
yaubwino woyamba. Zogulitsa zathu zapeza mbiri yabwino pamsika ndikudzidalira pakati pa makasitomala akale ndi akale ..