RNA Easy Fast Plant Tissue Kit

Kuyeretsa RNA yokwanira kuchokera kumatumba azomera.

Kitani cha RNA Easy Plant Tissue Kit ndi chida chofulumira cha RNA chopangira zida zazomera zopangidwa kutengera ukadaulo waukadaulo wa DNA wopangidwa ndi TIANGEN wokha. Ma reagents a poizoni monga β-mercaptoethanol kapena DTT safunika pantchito, ndipo ntchito yonse yochotsa RNA imatha kumaliza mphindi 30. Sikuti imangoyenera masamba azitsamba monga tirigu ndi chimanga, komanso zitsanzo za polysaccharide / polyphenol monga tsamba la Malus spectabilis, tsamba la thonje, tsamba la chrysanthemum, maluwa a hibiscus, tuber tuber, chivwende, nkhaka, singano ya paini , etc.

Mphaka. Ayi	Kukulitsa Kukula
4992880	50 preps

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Mankhwala Mwatsatanetsatane

Chitsanzo Cha Zoyesera

FAQ

Zogulitsa

Mawonekedwe

■ Zosavuta komanso zachangu: Kutulutsa kwathunthu kwa RNA pazitsanzo za mbeu kumatha kumaliza mphindi 30.
■ Kugwirizana kwamphamvu: Chikwama ichi sichimangoyenera zitsanzo za tsinde ndi masamba, komanso zitsanzo za polysaccharide / polyphenol.
■ Poizoni wotetezeka komanso wotsika: Ma reagents oopsa monga β-mercaptoethanol, DTT, chloroform, phenol safunika.

Mapulogalamu

■ Yoyenera kugwirira ntchito kutsika, kuphatikiza RT-PCR, RT-qPCR, chip hybridization, Northern Blot, Dot Blot, PolyA kuwunika, kumasulira kwa vitro, kusanthula chitetezo cha RNase ndikupanga ma molekyulu, ndi zina zambiri.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

Previous: Zamgululi RNA Easy Fast Tissue / Cell Kit

Ena: TGuide S32 Maginito Magazi a DNA Kit

	RNA yonse ya masamba 65 mg masamba (masamba a clove, masamba a hibiscus, masamba a tirigu, masamba a mpunga, masamba a zipatso za ugli, masamba a Prunus cerasifera, masamba a mkuyu, masamba a tsiku ndi tsiku, masamba a Kerria japonica), zitsanzo za bowa za 150 mg (Lentinus edode) ndi 200 Zitsanzo za mg petal (masamba a kakombo a tsiku ndi tsiku) adachotsedwa pogwiritsa ntchito zida za RNA Easy Fast Plant Tissue Kit.
	Agilent Bioanalyzer 2100 kusanthula zotsatira za RNA yathunthu kuchokera pamatumba a Day-kakombo.
	RNA yotulutsidwa pazitsanzo zingapo zomwe zalembedwa patebulo pogwiritsa ntchito Kiteni cha RNA Easy Fast Tissue Kit. Kuchepetsa mphamvu: 50 μl; Kutsegula voliyumu: 2 μl. Electrophoresis inkachitika pa 6 V / cm kwa mphindi 15 pa 1% agarose.

Q: Kutsekeka kwamagawo

A-1 Cell lysis kapena homogenization sikokwanira

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa kuchuluka kwa nyemba zomwe mukugwiritsa ntchito kapena kuonjezera kuchuluka kwa lysis buffer.

Q: Zokolola zochepa za RNA

A-1 Kusakwanira kwa cell lysis kapena homogenization

---- Kuchepetsa ntchito nyemba, kuonjezera kuchuluka kwa gawo lotetezera lysis, kuonjezera homogenization ndi nthawi lysis.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

- Chonde onani kuthekera kwakukulu kokonza.

A-3 RNA siyosankhidwa kwathunthu kuchokera pagawo

---- Mukatha kuwonjezera madzi opanda RNase, siyani kwa mphindi zochepa centrifuging.

A-4 Ethanol mumlengalenga

- Pambuyo kutsukidwa, centrifuge kachiwiri ndikuchotsa chotsuka chotsuka momwe zingathere.

A-5 Cell chikhalidwe sing'anga sichichotsedweratu

- Mukamasonkhanitsa maselo, chonde onetsetsani kuti muchotse sing'anga momwe mungathere.

A-6 Maselo osungidwa mu RNAstore sakhala otsekemera kwenikweni

---- RNAstore osalimba ndi wamkulu kuposa sing'anga wamba wamaselo; kotero mphamvu ya centrifugal iyenera kukulitsidwa. Akuti centrifuge pa 3000x g.

Zolemba za A-7 Low RNA komanso kuchuluka kwake mchitsanzo

- Gwiritsani ntchito chitsanzo chabwino kuti muwone ngati zokolola zochepa zimayambitsidwa ndi chitsanzocho.

Q: Kuwonongeka kwa RNA

A-1 Nkhaniyi siyatsopano

- Minyewa yatsopano imayenera kusungidwa mu nayitrogeni wamadzi nthawi yomweyo kapena kuyiyika mu reagent ya RNAstore kuti zitsimikizidwe kuti zimachokera.

A-2 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-3 RNase kuipitsidwan

- Ngakhale gawo lotetezedwa mulibe RNase, ndikosavuta kuipitsa RNase panthawi yazochulukirapo ndipo iyenera kusamalidwa.

Kuwononga kwa A-4 Electrophoresis

---- Sinthanitsani cholumikizira cha electrophoresis ndikuwonetsetsa kuti zofunikira ndi Kutsegula Buffer zilibe vuto la RNase.

A-5 Kutsitsa kwambiri kwa electrophoresis

---- Kuchepetsa kuchuluka kwa potsegula, kutsitsa kwa chitsime chilichonse sikuyenera kupitirira 2 μg.

Q: kuipitsidwa kwa DNA

A-1 Zitsanzo kuchuluka ndi waukulu kwambiri

---- Kuchepetsa nyemba kuchuluka.

A-2 Zitsanzo zina zimakhala ndi DNA yambiri ndipo amatha kuchiritsidwa ndi DNase.

- Chitani chithandizo cha RNase-Free DNase ku yankho la RNA, ndipo RNA itha kugwiritsidwa ntchito mwachindunji poyeserera pambuyo pothandizidwa, kapena itha kuyeretsedwa ndi zida zoyeserera za RNA.

Q: Kodi mungachotse bwanji RNase pazomwe mungagwiritse ntchito poyesera ndi magalasi?

Zamagalasi, zophikidwa pa 150 ° C kwa 4 h. Pazitsulo zapulasitiki, zomizidwa mu 0,5 M NaOH kwa mphindi 10, kenako kutsukidwa bwino ndi madzi opanda RNase kenako ndikuwotcha kuti muchotse RNase. Ma reagents kapena mayankho omwe amagwiritsidwa ntchito poyeserera, makamaka madzi, ayenera kukhala opanda RNase. Gwiritsani ntchito madzi opanda RNase pamakonzedwe onse a reagent (onjezerani madzi mu botolo loyera lagalasi, onjezerani DEPC pamlingo womaliza wa 0,1% (V / V), gwedezani usiku umodzi ndi autoclave).

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