Mu Disembala 2019, milandu ya chibayo ya zifukwa zosadziwika yayamba kuchokera ku Wuhan, Chigawo cha Hubei, ndi posakhalitsa anafalikira ku zigawo ndi mizinda yambiri ku China, ndi mayiko ena ambiri mu Januwale 2020. Kuyambira 22:00 pm pa Januware 27, 28 adatsimikizira milandu ndipo 5794 akuganiziridwa kuti milandu ya 2019-nCov idanenedwa ku China. Pulogalamu yaMatendawa amatengera Rhinolophus, ndipo anthu omwe amafa ndi 2.9%.
Pa Januware 12, 2020, World Health Organisation (WHO) yalengeza kuti mliri wa chibayo udayambitsidwa ndi mtundu watsopano wa coronavirus (2019-nCov). Coronavirus ndi mtundu wa kachilombo kamene kamafalikira pakati pa nyama. Vuto la nucleic acid ndi RNA imodzi. Nthawi yomweyo, WHO idatulutsanso chidziwitso cha nCov chotsatira cha nCov chogawana ndi akatswiri aku China, chomwe chapangitsa kuti maselo azindikire kuti ali ndi kachilombo ndipo zapangitsa kuti tizilombo toyambitsa matenda tizindikire mwachangu.
WHO ikuwonetsa kuti anthu omwe ali ndi matenda opatsirana kwambiri monga chifuwa, malungo, matenda opatsirana ndipo akhala ku Wuhan pasanathe masiku 14 kapena akumana ndi odwala ena ayenera kuyesedwa mokwanira. Pa 17 Januware 2020, WHO idatulutsa "Laboratory testing for 2019 novel coronavirus (2019-nCoV) mu milandu yomwe anthu akukayikira, Upangiri wanthawi yayitali, 17 Januware 2020". Malangizowo akuwonetsa kuti zitsanzo zomwe ziyenera kutengedwa kuchokera kwa odwala omwe ali ndi zidziwitso zimaphatikizapo zitsanzo za kupuma (nasopharynx ndi oropharyngeal swabs, sputum, bronchoalveolar lavage, ndi zina) ndi zitsanzo za seramu, motere:
* Pofuna kutumiza zitsanzo kuti muzindikire tizilombo toyambitsa matenda, gwiritsani ntchito VTM (ma virus oyendera ma virus) okhala ndi ma antifungal ndi maantibayotiki. Kwa chikhalidwe cha bakiteriya kapena fungal, mayendedwe owuma kapena ochepa kwambiri madzi osabala. Pewani kuzizira mobwerezabwereza ndikusungunula zitsanzo.
Kupatula pazinthu zakutolera zomwe zawonetsedwa patebulopo zimatsimikiziranso kuti zida ndi zida zina zilipo: mwachitsanzo, zotengera zoyendera ndi zikwama zosonkhanitsira zopangira ndi ma CD, zozizira ndi mapaketi ozizira kapena ayezi wouma, zida zosungira magazi zosabala (mwachitsanzo singano, majekeseni ndi machubu), Zolemba ndi zolembera zokhazikika, PPE, zida zowonongera malo.
Kuti muzindikire ma virus, ma serum specimens atha kugwiritsidwa ntchito kuzindikira kachilombo pogwiritsa ntchito njira yoteteza thupi, pomwe RTqPCR ikulimbikitsidwa kuti izindikire nCov nucleic acid kuti izindikire mwachangu komanso molondola. Monga kachilombo
Mndandanda umadziwika, zomwe zili ndi ma virus a nucleic acid mu fanizoli limatha kupezeka mwachangu kokha
kusankha ma reagents oyenera ndi zoyambira zofananira
(Kuzindikira matenda a Wuhan coronavirus 2019 ndi nthawi yeniyeni ya RTPCR, Protocol ndi kuwunika koyambirira kuyambira pa Jan 13, 2020) Monga mtsogoleri pantchito yotulutsa ndi kupeza asidi ku China, TIANGEN BIOTECH (BEIJING) CO., LTD. yapereka kachilombo koyambitsa matendawa ndi fuluwenza A (H1N1) yothetsera ma reagents kwa anthu opitilira 10 miliyoni. Mu 2019, TIANGEN's virus nucleic acid extraction and detection reagents agwiritsidwa ntchito ku 30 miliyoni African swine fever sampuli, zomwe zathandizira kwambiri pakuzindikira ndi kupewa kwa African classical swine fever ku China. TIANGEN sikuti imangotulutsa mwachangu komanso molondola ma reagents a kachilombo, komanso imaperekanso zida zopangira minofu yabwino komanso zotsekera ma nucleic acid, zomwe zimapanga mayankho athunthu pakuchotsa ma virus ndikudziwika.
Yodzichitira Nucleic Acid m'zigawo Anakonza
TIANGEN Yodzichitira Yokha Nucleic Acid Extractors ndi nsanja zodziwikiratu za kuchotsera acid acid pogwiritsa ntchito maginito mkanda njira. Kugwiritsa ntchito nsanjazi sikuti kumangochepetsa kuchuluka kwa magwiridwe oyang'anira ndi kuyika anthu m'madipatimenti, komanso kumachepetsa zolakwika pakuwongolera pamanja, ndikuwonetsetsa kusasinthasintha ndi kukhazikika kwa mtundu wa asidi wa asidi.
TIANGEN Automated Nucleic Acid Extractor ili ndi njira zosiyanasiyana (kuphatikiza njira 16, 24, 32, 48, 96), ndipo ma reagents omwe angagwiritsidwe ntchito atha kugwiritsidwa ntchito popanga ma nucleic acid kuchokera kuzitsanzo zosiyanasiyana. TIANGEN imaperekanso ntchito zosinthika za reagent ndi zida zophatikizira malinga ndi zofunikira zosiyanasiyana zoyesera.
TGrinder H24 minofu homogenizer
● Amagwiritsidwa ntchito kwambiri popera ndi kusungunula mitundu yosiyanasiyana ya ziwalo ndi ndowe
● Pogaya homogenization mphamvu nthawi 2-5 kuposa zida zachikhalidwe
● Kupera yunifolomu ndi homogenization, kupewa kuwonongeka kwa mtanda
● Chida chodzitetezera chokha kuti chiteteze ogwira ntchito labotale
TGuide S32 Yodzichitira Nucleic Acid Sola
● Kutulutsa kwazitsanzo: zitsanzo 1-32
● Kuchepetsa voliyumu: 20-1000 μl
● Mtundu wa Zitsanzo: Magazi, maselo, minofu, ndowe, mavairasi ndi zina
● Nthawi yokonza: Mpaka mphindi 8 kuti mupeze ma virus a acid
● Njira Yoyang'anira: Mawindo awiri owongolera a Windows Pad ndi batani pazenera
● Kukula kwa nsanja: Pulatifomu yotseguka, yaulere mofanana ndi ma reagents
● TGuide S32 Magnetic Viral DNA / RNA Kit (DP604)
● Kugwiritsa ntchito kwambiri: DNA / RNA yamafuta abwino kwambiri imatha kutsukidwa ku seramu, plasma, swab sampuli, njira yothandizira minofu ndi njira zingapo zotetezera ma virus.
● Zosavuta komanso zothandiza: Chogulitsachi chikugwirizana bwino ndi TGuide S32 Automated Nucleic Acid Extractor, yomwe imatha kutulutsa kachilombo ka DNA / RNA ndi zokolola zambiri, chiyero chambiri, khola komanso khalidwe lodalirika.
● Kugwiritsa ntchito kumunsi kwa madzi: Nucleic acid yoyeretsedwa ndiyabwino poyesera kutsika kwa kachilombo ka PCR ndi PCR yeniyeni.
Buku Nucleic Acid Extraction Solution
Monga mtsogoleri pankhani yopanga ndi kuzindikira asidi, TIANGEN ali ndi mndandanda wazinthu zambiri zopangira ma acid ku China, zomwe ndizofunikira pakuwunika kwa coronavirus kuchokera ku mitundu yonse yazitsanzo: magazi, seramu / plasma, swab, virus , etc.
TIANamp Virus DNA / RNA Kit (DP315)
● Kuchita bwino kwambiri: Ma virus a DNA ndi RNA amatha kupezeka mwa kuyeretsa mwachangu mobwerezabwereza.
● Kuyera kwambiri: Kuchotsa kwathunthu zoipitsa ndi zotsekereza kuti zitsike kumapeto.
● Chitetezo chachikulu: Kutulutsa kwa reagent kapena mpweya wa ethanol sikofunikira.
Njira Yothetsera Vuto la RNA
1. Makina Osewera Pipetting
● Kulondola kwambiri: Malo ozizira amatha kusunga kutentha kwa reagent pansipa 7 ℃ kwa mphindi 60. APM iliyonse imayang'aniridwa molingana ndi miyezo yapadziko lonse lapansi, yomwe imakhala yolondola kwambiri kuposa kupopera kwa manja.
● Ntchito yosavuta: Kukula pang'ono. Kulemera pang'ono. Palibe chida chofunikira pakulowetsa m'malo mwa block. Ndondomeko yokonzekera PCR / qPCR yokonzekera. Ntchito yosavuta yokonzekera yankho la PCR.
● Kugwiritsa ntchito kwambiri: Itha kugwiritsidwa ntchito popopera mapaipi a zitsime za 96/384, PCR, qPCR, kuzindikira majini ndi zoyeserera zina zapamwamba.
FastKing Gawo Lina RT-qPCR Kit (Probe) (FP314)
FastKing One Step RT-qPCR Kit (Probe) (FP314) yopangidwa ndi TIANGEN ndi chida chodziwitsira chowunikira chowerengera cha fluorescence potengera njira yofufuzira, yomwe idapangidwa kuti ipeze mitundu yazomwe ikuyang'ana mumitundu yosiyanasiyana. The KingRTase mu kit ndi ma molekyulu atsopano osinthidwa reverse transcriptase, omwe ali ndi mphamvu yolimba ya RNA komanso kukhazikika kwamatenthedwe, ndikuwongolera bwino magwiridwe antchito ndikuwonjezera kuthekera kwa ma templates ovuta a RNA. Chiyambi chatsopano cha Taq DNA polymerase chimagwiritsidwanso ntchito kupatsa mphamvu ya PCR kukweza kwamphamvu komanso kulunjika. Kuphatikiza apo, zida zimathandizira kuti zinthuzo zikhale zocheperako kwambiri posakaniza ma enzyme a Taq ndi MLV mu kusakaniza ma enzyme, komanso kusakaniza pre-ion buffer, dNTPs, PCR stabilizer ndikupititsa patsogolo MasterMix, kuti njira zosakanikirana zingapo zitha kukhala
chosavuta.
● Kuchita bwino kwambiri: The reverse reverse transcriptase ndi DNA polymerase zimawonetsetsa kuti magwiridwe antchito bwino
● Kusintha kwabwino: Polymerase imatha kuwerengera template ya RNA yokhala ndi ma GC apamwamba komanso mawonekedwe ena ovuta
● Kugwiritsa ntchito kwambiri: Kugwiritsa ntchito kwambiri ma templeti a RNA okhala ndi zodetsa zamitundu yosiyanasiyana
● Kuzindikira kwambiri: Ma tempuleti otsika ngati 1 ng amatha kuzindikirika molondola, makamaka pazithunzi zochepa
Chitsanzo cha Kuzindikira Kachilombo ka RNA
Nucleic acid ya H5 avian fuluwenza idapangidwa ndi TGuide S32 Magnetic Viral DNA / RNA Kit (DP604). FastKing One Step RT-qPCR Kit (Probe) (FP314) idagwiritsidwa ntchito pozindikira RT-qPCR pogwiritsa ntchito njira zoyambira ndi fuluwenza za H5 avian fuluwenza.
ABi7500Fast idagwiritsidwa ntchito pozindikira RT-qPCR. Zotsatira za H5 avian fuluwenza virus antigen (10-2, 10-3, 10-4, 10-5, 10-6and 10-7 dilution) kuchokera ku 200 μl zitsanzo zikuwonetsa kuchuluka kwa ma virus a nucleic acid, omwe amatha kuthana ndi zotsatira zake zosowa zakulemba kosinthika, PCR, RT-PCR, PC-real-time, ndi zina. PC-real-time imapereka zotsatira ndikumverera kwakukulu, kubwerezabwereza bwino komanso kulumikizana kwabwino kwa gradient. Mitundu yosiyanasiyana ya mitundu ya ma virus ya acid ikhoza kudziwika bwino.
Post nthawi: Apr-11-2021
