FastKing Gawo Limodzi la RT-qPCR kit

Gawo limodzi la Real-Time RT-PCR yokhala ndi SYBR-Green.

FastKing One Step RT-qPCR Kit idapangidwa kuti ichitepo kanthu pa Real-Time One Step RT-qPCR pogwiritsa ntchito SYBR Green I. Chidacho chitha kugwiritsidwa ntchito pompopompo pa RT-qPCR pazolinga za RNA, ndikulemba kosinthika ndi PCR kumachitika motsatana mu chubu chomwecho. Chifukwa chake opaleshoniyi ndi yosavuta, ndipo imachepetsa chiopsezo cha kuipitsidwa.

The 25 × RT-PCR Enzyme Mix in this kit is a premix of TIANGEN's novel reverse transcriptase (FastKing RTase), anti antibody yosinthidwa hotstart Taq DNA polymerase ndi RNase Inhibitor. FastKing RTase ndi mtundu watsopano wama cell osinthidwa reverse transcriptase. Ndi mawonekedwe owonjezera a hydrophobic, omwe ali ndi mphamvu yolimba ya RNA komanso kukhazikika kwamatenthedwe. FastKing RTase yasintha magwiridwe antchito osinthira komanso kuthekera kokulitsa template ya RNA yokhala ndi mawonekedwe ena ovuta. Mtundu watsopano wa hotstartTaq DNA polymerase yokhala ndi magwiridwe antchito imagwiritsidwa ntchito pokonza PCR, kupangitsa zomwe PCR kuchita pambuyo polemba kusindikiza kukhala ndi kukweza kwapamwamba kwambiri komanso mwatsatanetsatane. Kuphatikiza apo, 2 × FastKing RT-qPCR Buffer (SYBR Green) muchinthu ichi ndi njira yatsopano yopangira ma enzyme awiriwa, kuphatikiza ma ionic ofunikira, dNTPs, utoto wa SYBR Green, sitepe imodzi yolimbitsa ndi yopititsira patsogolo, yomwe zitha kuwonetsetsa kuti FastKing RTase ndi hotstart yatsopanoTaq DNA polymerase imatha kugwira ntchito yofunikira kwambiri pakuchita gawo limodzi.

Chida ichi chimakhala ndi ma curve abwino m'malo osiyanasiyana. Ikhoza kuzindikira molondola komanso kuchuluka kwa mitundu yambiri yazotsika ndi yotsika yomwe imakhala ndi kubwereza bwino komanso kudalirika kwambiri.