Chida cha EndoFree Maxi Plasmid V2

Kuyeretsedwa kwa endotoxin-free transfection grade plasmid DNA yodziwika ndi ma cell osazindikira.

EndoFree Maxi Plasmid Kit V2 imagwiritsa ntchito njira yapadera yolumikizira silika kuti igwiritse bwino ntchito komanso kuphatikiza DNA ya plasmid. Buffer ER yapadera ndi Filtration CS1 imatha kuchotsa zosafunika monga endotoxin ndi protein. Njira yonse yochotsera imatenga ola limodzi lokha.
Analimbikitsa mabakiteriya chikhalidwe chamkati kuchuluka kwa plasmid, ma 100 ml mabakiteriya amalimbikitsidwa, omwe amatha kutulutsa mpaka 500-1500 μg plasmid. Kwa plasmid yotsika pang'ono, 200 ml ya mabakiteriya sing'anga amalimbikitsidwa kuti akwaniritse zokolola pafupifupi 50-300 μg.

Mphaka. Ayi	Kukulitsa Kukula
4992438	10 ma preps

Tumizani imelo kwa ife

Mankhwala Mwatsatanetsatane

Kayendedwe

Chitsanzo Cha Zoyesera

Zogulitsa

Mawonekedwe

■ Kuyera kwambiri: Ukadaulo wapadera wa endotoxin mpweya umalandiridwa kuti uchotsere endotoxin.
■ Yosavuta kuyigwiritsa ntchito: Ukadaulo wazipangizo zotsatsira anthu umagwiritsidwa ntchito kutsatsa mwachindunji plasmid DNA, zomwe zimapangitsa kuti ntchitoyo ikhale yosavuta.
■ Kupititsa patsogolo kwambiri: Koyenera kusanjikiza kwa mizere yambiri yamaselo kuphatikiza maselo ozindikira endotoxin.
■ Kugwiritsa ntchito kosiyanasiyana: Plasmid yoyeretsedwa itha kugwiritsidwa ntchito posamutsa nyama ndi maselo am'magulu komanso zoyeserera za biology.

Mapulogalamu

DNA ya plasmid yotulutsidwa pogwiritsa ntchito chida ichi itha kugwiritsidwa ntchito pochita zinthu zosiyanasiyana, kuphatikiza chimbudzi chothandizira, PCR, kusanja, ligation, kusintha ndikusinthitsa kwa maselo osiyanasiyana.

Zogulitsa zonse zimatha kusinthidwa kukhala ODM / OEM. Zambiri,chonde dinani Makonda Service (ODM / OEM)

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Gawo lapadera la endotoxin kuchotsa reagent Buffer ER limatha kuchotsa bwino zotsalira za endotoxin mumachitidwe, ndipo plasmid yoyera kwambiri imatha kupezeka. Zotsalira za plasmid endotoxin ndi ≤ 0.1 EU / ml.

Plasmid yoyeretsedwa pogwiritsa ntchito EndoFree Maxi Plasmid Kit V2 ndi zinthu zomwezo kuchokera ku Supplier 1 ndi Supplier 2 zili ndi voliyumu yofananira ya elution. 1 μl plasmid idayikidwa pamseu uliwonse kuyerekezera kuchuluka kwa plasmid. 100 ng ya plasmid yemwe ndende yake idatsimikiziridwa ndi spectrophotometer idayikidwa mu gel yomweyo kuti mudziwe ngati kuchuluka kwake kumakhala konyenga kwambiri.
Kutsiliza: Zotsatira za electrophoresis zikuwonetsa kuchuluka kwa plasmid yotulutsidwa ndi chida cha EndoFree Maxi Plasmid V2, ngakhale chokwanira kapena chotsika, ndichokwera kwambiri kuposa chomwe chimaperekedwa ndi ogulitsa 1 ndi 2.

Lembani uthenga wanu apa ndi kutumiza kwa ife